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研究業績詳細

木南英紀（コミナミエイキ）

研究テーマ	タンパク質の分解機構
研究業績（論文）	(2000―2009年）　英文原著 1. Ichimura, Y., Kirisako, T., Takao, T., Satomi, Y., Shimonishi, Y., Ishihara, N., Mizushima N., Tanida, I., Kominami, E., Ohsumi, M., Noda, T., Ohsumi, Y. ：A ubiquitin-like system mediates protein lipidation . Nature, 408, 488-492 (2000) 2. Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami,E, Ohsumi, Y., Yoshimori, T.: LC3, a mammalian homolog of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J., 19, 5720-5728 (2000) 3. Gotow T, Shibata M, Kanamori S, Tokuno O, Ohsawa Y, Sato N, Isahara K, Yayoi Y, Watanabe T, Leterrier JF, Linden M, Kominami E, Uchiyama Y: Selective localization of Bcl-2 to the inner mitochondrial and smooth endoplasmic reticulum membranes in mammalian cells. Cell Death Differ., 7, 666-674 (2000) 4. Takano-Ohmuro, H., Mukaide, M., Kominami, E., Morioka, K. : Autophagy in embryonic erythroid cells: its role in maturation. Eur. J. Cell Biol., 79: 759-764 (2000) 5. Koike, M., Nakanishi, H., Saftig, P., Ezaki, J., Isahara, K., Ohsawa, Y., Schulz-Schaeffer, W., Watanabe, T., Waguri, S., Kametaka, S., Shibata, M., Yamamoto, K., Kominami, E., Peters, C., von Figura, K., Uchiyama, Y.: Cathepsin D Deficiency Induces Lysosomal Storage with Ceroid Lipofuscin in Mouse CNS Neurons. J. Neurosci., 15, 6898-6906 (2000) 6. Ezaki J, Takeda-Ezaki M, Oda K, and Kominami E.: Characterization of endopeptidase activity of tripeptidyl peptidase-I/ CLN2 protein which is deficient in classical late infantile neuronal ceroid lipofuscinosis Biochem. Biophys.Res.Commun., 268, 904-908 (2000) 7. Ezaki, J., Takeda-Ezaki, M., Kominami, E.: Tripeptidyl peptidase I, the late infantile neuronal ceroid lipofuscinosis gene product, initiates the lysosomal degradation of subunit c of ATP synthase. J. Biochem., 128, 509 – 516 (2000) 8. Suopanki, J., Partanen, S., Ezaki, J., Baumann, M., Kominami, E.TyynelｸJ・ J.: Developmental changes in the expression of NCL-linked proteins. Mol. Genet.Metab., 71, 190-194 (2000) 9. Okazaki, N., Yan, J., Yuasa, S., Ueno, T., Kominami, E., Masuho, Y., Koga, H., Muramatsu, M.: Interaction of Unc-51-like kinase and MAP light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation. Mol. Brain. Res., 85, 1-12 (2000) 10. Iida, T., Nakaseko, C., Ohno, H., Sakuma, M., Ezaki-Takeda, M., Kominami, E., Fujisawa, T., Saito, T.: Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4 containing l ysosomes upon activation of CD4+T cells. J. Immunol., 165, 5062-5068 (2000) 11. Muno, D., Kominami, E., Mizuochi, T.: Generation of both MHC class I- and class II-restricted antigenic peptides from exogenously added ovalbumin in murine phagosomes. FEBS Lett., 478, 178-182 (2000) 12. Kasai, M., Kropshofer, H., Vogt, AB., Kominami, E., Mizuochi, T.: CLIP-derived self peptides bounds to MHC class II molecules of medullary thymic epithelial cells differ from those of cortical thymic epithelial cells In their diversity, length, and C-terminal processing. Eur. J. Immunol., 30, 3542-3551 (2000) 13. Kawada, A., Hara, K., Kominami, E., Tezuka, T., Takahashi, M., Takahara, H.: Precursor of rat epidermal cathepsin L: purification and immunohistochemical localization. J. Dermatol. Sci., 23, 36-45 (2000) 14. Tanida, I., Tanida-Miyake, E., Ueno, T., Kominami, E.:The Human homologue of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates, human Apg12p, GATE-16, GABARAP, and MAP-LC3. J. Biol. Chem., 276, 1701-1706 (2001) 15. Komatsu, M., Tanida, I., Ueno, T., Kiyooka, M., Mariko Ohsumi, M., Ohsumi,Y. Kominami, E.: Dimerization of Apg7p/Cvt2p is essential for E1 activity and interaction with Aut7p/Apg8p and Aut1p/Apg3p. J.Biol. Chem., 276, 9846-9854 (2001) 16. Furuya N, Kanazawa T, Fujimura S, Ueno T, Kominami E, Kadowaki M: Leupeptin-induced appearance of partial fragment of betaine homocysteine methyltransferase during autophagic maturation in rat hepatocytes. J Biochem., 129, 313-320 (2001) 17. Goebel HH, Kominami E, Neuen-Jacob E, Wheeler RB: Morphological studies on CLN2. Europ J Paediatr Neurol. 5 Suppl A:203-207 (2001) 18. Kaneko M, Tomita T, Nakase T, Ohsawa Y, Seki H, Takeuchi E, Takano H, Shi K, Takahi K, Kominami E, Uchiyama Y, Yoshikawa H, Ochi T.: Expression of proteinases and inflammatory cytokines in subchondral bone regions in the destructive joint of rheumatoid arthritis. Rheumatology, 40, 247-255 (2001) 19. Kim BY, Kramer H, Yamamoto A, Kominami E, Kohsaka S, Akazawa C: Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7. J Biol Chem., 276, 29393-29402 (2001) 20. Nakanishi H, Zhang J, Koike M, Nishioku T, Okamoto Y, Kominami E, von Figura K, Peters C, Yamamoto K, Saftig P, Uchiyama Y: Involvement of nitric oxide released from microglia- macrophages in pathological changes of cathepsin D-deficient mice. J Neurosci. 21, 7526-7533 (2001) 21. Shibata M, Kanamori S, Ohsawa Y, Watanabe T, Yayoi Y, Miura M, Kominami E, Uchiyama Y: Prevention of apoptosis of mammalian cells by the CED-3-cleaved form of CED-9. Arch Histol Cytol., 64, 17-28 (2001) 22. Uchida K, Ohmori D, Ueno T, Nishizuka M, Eshita Y, Fukunaga A, Kominami E: Preoviposition activation of cathepsin-like proteinases in degenerating ovarian follicles of the mosquito Culex pipiens pallens. Dev Biol., 237, 68-78 (2001) 23. Waguri S, Kohmura M, Kanamori S, Watanabe T, Ohsawa Y, Koike M, Tomiyama Y, Wakasugi M, Kominami E, Uchiyama Y: Different distribution patterns of the two mannose 6-phosphate receptors in rat liver. J Histochem Cytochem. 49, 1397-1406 (2001) 24. Yayoi Y, Ohsawa Y, Koike M, Zhang G, Kominami E, Uchiyama Y: Specific localization of lysosomal aminopeptidases in type II alveolar epithelial cells of the rat lung. Arch Histol Cytol. 64, 89-97 (2001) 25. Zhang T, Maekawa Y, Sakai T, Nakano Y, Ishii K, Hisaeda H, Kominami E, Katunuma N, Asao T, Himeno K: Splenic cathepsin L is maturated from the proform by interferon-gamma after immunization with exogenous antigens. Biochem Biophys Res Commun. 283, 499-506 (2001) 26. Wheeler RB, Schlie M, Kominami E, Gerhard L, Goebel HH: Neuronal ceroid lipofuscinosis: late infantile or Jansky Bielschowsky type--re-revisited. Acta Neuropathol . 102, 485-488 (2001) 27. Ishidoh K, Kominami E.: Processing and activation of lysosomal proteinases.Biol Chem. 383(12), 1827-1831 (2002) 28. Koike M, Shibata M, Ohsawa Y, Kametaka S, Waguri S, Kominami E, Uchiyama Y.: The expression of tripeptidyl peptidase I in various tissues of rats and mice. Arch Histol Cytol. 65(3), 219-232 (2002) 29. Tanida I, Nishitani T, Nemoto T, Ueno T, Kominami E.: Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing. Biochem Biophys Res Commun. 296(5), 1164-1170 (2002) 30. Yoshida M, Yamashima T, Zhao L, Tsuchiya K, Kohda Y, Tonchev AB, Matsuda M, Kominami E.: Primate neurons show different vulnerability to transient ischemia and response to cathepsin inhibition. Acta Neuropathol , 104(3), 267-272 (2002) 31. Asanuma K, Shirato I, Ishidoh K, Kominami E, Tomino Y.: Selective modulation of the secretion of proteinases and their inhibitors by growth factors in cultured differentiated podocytes. Kidney Int., 62, 822-831 (2002) 32. Yoshikawa M, Uchida S, Ezaki J, Rai T, Hayama A, Kobayashi K, Kida Y, Noda M, Koike M, Uchiyama Y, Marumo F, Kominami E, Sasaki S.: CLC-3 deficiency leads to phenotypes similar to human neuronal ceroid lipofuscinosis. Genes Cells. 7, 597-605 (2002) 33. Kouroku Y, Fujita E, Jimbo A, Kikuchi T, Yamagata T, Momoi MY, Kominami E, Kuida K, Sakamaki K, Yonehara S, Momoi T. : Polyglutamine aggregates stimulate ER stress signals and caspase-12 activation. Hum Mol Genet. 11, 1505-1515, 2002 34. Shibuya S, Wakayama Y, Inoue M, Oniki H, Kominami E. : Changes in the distribution and density of caveolin 3 molecules at the plasma membrane of mdx mouse skeletal muscles: a fracture-label electron microscopic study. Neurosci Lett. 325, 171-174 (2002) 35. Tanida I, Tanida-Miyake E, Nishitani T, Komatsu M, Yamazaki H, Ueno T, Kominami E.: Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs. Biochem Biophys Res Commun., 292, 256-262 (2002) 36. Tanida I, Tanida-Miyake E, Komatsu M, Ueno T, Kominami E. : Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J Biol Chem., 277, 13739-13744 (2002) 37. Tanida I, Komatsu M, Ueno T, Kominami E.: GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3. Biochem Biophys Res Commun., 300, 637-644 (2003) 38. Koike M, Shibata M, Ohsawa Y, Nakanishi H, Koga T, Kametaka S, Waguri S, Momoi T, Kominami E, Peters C, Figura K, Saftig P, Uchiyama Y.: Involvement of two different cell death pathways in retinal atrophy of cathepsin D-deficient mice. Mol Cell Neurosci., 22, 146-161, (2003) 39. Asanuma K, Tanida I, Shirato I, Ueno T, Takahara H, Nishitani T, Kominami E, Tomino Y.: MAP-LC3, a promising autophagosomal marker, is processed during the differentiation and recovery of podocytes from PAN nephrosis. FASEB J., 17, 1165-1167 (2003) 40. Haraguchi CM, Ishido K, Kominami E, Yokota S.: Expression of cathepsin H in differentiating rat spermatids: immunoelectron microscopic study. Histochem Cell Biol., 120, 63-71 (2003) 41. Katoh K, Shibata H, Suzuki H, Nara A, Ishidoh K, Kominami E, Yoshimori T, Maki M.: The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting. J Biol Chem. 278, 39104-39113 (2003) 42. Nemoto T, Tanida I, Tanida-Miyake E, Minematsu-Ikeguchi N, Yokota M, Ohsumi M, Ueno T, Kominami E.: The mouse APG10 homologue, an E2-like enzyme for Apg12p conjugation, facilitates MAP-LC3 modification. J Biol Chem. 278, 39517-39526 (2003) 43. Yamazaki-Sato H, Tanida I, Ueno T, Kominami E.: The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation. FEBS Lett. 551, 71-77 (2003) 44. Yamashima T, Tonchev AB, Tsukada T, Saido TC, Imajoh-Ohmi S, Momoi T, Kominami E.: Sustained calpain activation associated with lysosomal rupture executes necrosis of the postischemic CA1 neurons in primates. Hippocampus., 13, 791-800 (2003) 45. Ezaki J, Takeda-Ezaki M, Koike M, Ohsawa Y, Taka H, Mineki R, Murayama K, Uchiyama Y, Ueno T, Kominami E.: Characterization of Cln3p, the gene product responsible for juvenile neuronal ceroid lipofuscinosis, as a lysosomal integral membrane glycoprotein. J Neurochem. 87, 1296-1308 (2003) 46. Kim BY, Ueda M, Kominami E, Akagawa K, Kohsaka S, Akazawa C.: Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex., Biochem Biophys Res Commun. 311, 577-582 (2003) 47. Tsiakas K, Steinfeld R, Storch S, Ezaki J, Lukacs Z, Kominami E, Kohlschutter A, Ullrich K, Braulke T.: Mutation of the glycosylated asparagine residue 286 in human CLN2 protein results in loss of enzymatic activity. Glycobiology. 14, 1C-5C (2004) 48. Komatsu M, Chiba T, Tatsumi K, Iemura S, Tanida I, Okazaki N, Ueno T, Kominami E, Natsume T, Tanaka K.: A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier. EMBO J., 23, 1977-1986 (2004) 49. Tanida I, Sou YS, Ezaki J, Minematsu-Ikeguchi N, Ueno T, Kominami E.: HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates. J Biol Chem., 279, 36268-36276 (2004) 50. Reiser J, Oh J, Shirato I, Asanuma K, Hug A, Mundel TM, Honey K, Ishidoh K, Kominami E, Kreidberg JA, Tomino Y, Mundel P.: Podocyte migration during nephrotic syndrome requires a coordinated interplay between cathepsin L and alpha3 integrin. J Biol Chem. 279, 34827-34832., (2004) 51. Kouno T, Mizuguchi M, Tanida I, Ueno T, Kominami E, Kawano K.: 1H, 13C, and 15N resonance assignments of human microtubule-associated protein light chain-3. J Biomol NMR., 29(3):415-416 (2004) 52. Lecaille F, Muno D, Kominami E, Ishidoh K.: Proteinases participating in the processing and activation of prolegumain in primary cultured rat macrophages. Biol Chem., 385, 511-516 (2004) 53. Jager S, Bucci C, Tanida I, Ueno T, Kominami E, Saftig P, Eskelinen EL.: Role for Rab7 in maturation of late autophagic vacuoles. J Cell Sci., 117:4837-4848 2004 54. Tanida I, Ueno T, Kominami E.: Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes. J Biol Chem. 279, 47704-47710 (2004) 55. Komatsu M, Waguri S, Ueno T, Iwata J, Fujimura T, Murata S, Tanida I, Ezaki J, Mizushima N, Ohsumi Y, Kurayama K, Uchiyama Y, Kominami E, Tanaka K, Chiba T : Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol, 169(3), 425-434 (2005) 56. Tanida I, Minematsu-Ikegami N, Ueno T, Kominami E.: Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy, 1, 84-91 (2005) 57. Koike M, Shibata M, Waguri S, Yoshimura K, Tanida I, Kominami E, Gotow T, Peters C, von Figura K, Mizushima N, Saftig P, Uchiyama Y.: Participation of autophagy in storage of lysosomes in neurons from mouse models of neuronal ceroid-lipofuscinoses (Batten disease). Am J Pathol. 167(6):1713-1728 (2005) 58. Kouno T, Mizuguchi M, Tanida I, Ueno T, Kanematsu T, Mori Y, Shinoda H, Hirata M, Kominami E, Kawano K.: Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains. J Biol Chem. 280(26), 24610-24607 (2005) 59. Sou YS, Tanida I, Komatsu M, Ueno T, Kominami E. : Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16. J.Biol Chem. 281(6):3017-3024 (2006) 60. Iwata J, Ezaki J, Komatsu M, Yokota S, Ueno T, Tanida I, Chiba T, Tanaka K, Kominami E.: Excess peroxisomes are degraded by autophagic machinery in mammals. J Biol Chem., 281(7):4035-4041 (2006) 61. Kumanomidou T, Mizushima T, Komatsu M, Suzuki A, Tanida I, Sou YS, Ueno T, Kominami E, Tanaka K, Yamane T. : The crystal structure of human Atg4b, a processing and de-conjugating enzyme for autophagosome-forming modifiers. J Mol Biol. 355(4):612-618. (2006) 62. Sasakawa H, Sakata E, Yamaguchi Y, Komatsu M, Tatsumi K, Kominami E, Tanaka K, Kato K.: Solution structure and dynamics of Ufm1, a ubiquitin-fold modifier 1.　Biochem Biophys Res Commun. 343(1):21-26.（2006） 63. Komatsu M, Waguri S, Chiba T, Murata S, Iwata J, Tanida I, Ueno T, Koike M, Uchiyama Y, Kominami E, Tanaka K.: Loss of autophagy in the central nervous system causes neurodegeneration in mice.　Nature. 441(7095):880-884. （2006） 64. Tanida I, Sou YS, Minematsu-Ikeguchi N, Ueno T, Kominami E.: Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3.　FEBS J. 273(11):2553-2562. （2006） 65. Kouroku Y, Fujita E, Tanida I, Ueno T, Isoai A, Kumagai H, Ogawa S, Kaufman RJ, Kominami E, Momoi T.: ER stress (PERK/eIF2alpha phosphorylation) mediates the polyglutamine-induced LC3 conversion, an essential step for autophagy formation. Cell Death Differ. 14(2):230-239. (2006 ) 66. Tanida I, Wakabayashi M, Kanematsu T, Minematsu-Ikeguchi N, Sou YS, Hirata M, Ueno T, Kominami E. : Lysosomal turnover of GABARAP-phospholipid conjugate is activated during differentiation of C2C12 cells to myotubes without inactivation of the mTor kinase-signaling pathway. Autophagy. 2(4):264-271. (2006) 67. Komatsu M, Kominami E, Tanaka K. : Autophagy and neurodegeneration. Autophagy. 2(4):315-317. (2006) 68. Yogosawa S, Kawasaki M, Wakatsuki S, Kominami E, Shiba Y, Nakayama K, Kohsaka S, Akazawa C. : Monoubiquitylation of GGA3 by hVPS18 regulates its ubiquitin-binding ability. Biochem Biophys Res Commun. 350(1):82-90. (2006) 69. Kim BY, Sahara Y, Yamamoto A, Kominami E, Kohsaka S, Akazawa C. : The interaction of mammalian Class C Vps with nSec-1/Munc18-a and syntaxin 1A regulates pre-synaptic release. Biochem Biophys Res Commun. 350(3):691-697. (2006 ) 70. Persaud-Sawin DA, Mousallem T, Wang C, Zucker A, Kominami E, Boustany RM. : Neuronal ceroid lipofuscinosis: a common pathway? Pediatr Res. 61(2):146-152. (2007) 71. Yamatake K, Maeda M, Kadowaki T, Takii R, Tsukuba T, Ueno T, Kominami E, Yokota S, Yamamoto K. : Role for gingipains in Porphyromonas gingivalis traffic to phagolysosomes and survival in human aortic endothelial cells. Infect Immun. 75(5):2090-2100. (2007) 72. Mizokami A, Kanematsu T, Ishibashi H, Yamaguchi T, Tanida I, Takenaka K, Nakayama KI, Fukami K, Takenawa T, Kominami E, Moss SJ, Yamamoto T, Nabekura J, Hirata M. : Phospholipase C-related inactive protein is involved in trafficking of gamma2 subunit-containing GABA(A) receptors to the cell surface. J Neurosci. 27(7): 1692-1701 (2007) 73. Komatsu M, Wang QJ, Holstein GR, Friedrich VL Jr, Iwata J, Kominami E, Chait BT, Tanaka K, Yue Z. : Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration. Proc Natl Acad Sci U S A. 104(36):14489-14494. (2007) 74. Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T. : Crystal structure of Ufc1, the Ufm1-conjugating enzyme. Biochem Biophys Res Commun. 362(4):1079-1084. (2007 ) 75. Komatsu M, Waguri S, Koike M, Sou YS, Ueno T, Hara T, Mizushima N, Iwata J, Ezaki J, Murata S, Hamazaki J, Nishito Y, Iemura S, Natsume T, Yanagawa T, Uwayama J, Warabi E, Yoshida H, Ishii T, Kobayashi A, Yamamoto M, Yue Z, Uchiyama Y, Kominami E, Tanaka K. : Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell. 131(6):1149-1163.(2007) 76. Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K. : Consideration about negative controls for LC3 and expression vectors for four colored fluorescent protein-LC3 negative controls. Autophagy. 4(1)131-134. (2008) 77. Koike M, Shibata M, Tadakoshi M, Gotoh K, Komatsu M, Waguri S, Kawahara N, Kuida K, Nagata S, Kominami E, Tanaka K, Uchiyama Y.: Inhibition of autophagy prevents hippocampal pyramidal neuron death after hypoxic-ischemic injury. Am J Pathol. 172(2):454-469. (2008) 78. Matsumoto N, Ezaki J, Komatsu M, Takahashi K, Mineki R, Taka H, Kikkawa M, Fujimura T, Takeda-Ezaki M, Ueno T, Tanaka K, Kominami E. : Comprehensive proteomics analysis of autophagy-deficient mouse liver. Biochem Biophys Res Commun. 368(3):643-649. (2008) 79. Ueno T, Sato W, Horie Y, Komatsu M, Tanida I, Yoshida M, Ohshima S, Mak TW, Watanabe S, Kominami E. : Loss of Pten, a tumor suppressor, causes the strong inhibition of autophagy without affecting LC3 lipidation. Autophagy. 4(5):692-700. (2008) 80. Ichimura Y, Kumanomidou T, Sou YS, Mizushima T, Ezaki J, Ueno T, Kominami E, Yamane T, Tanaka K, Komatsu M. : Structural basis for sorting mechanism of p62 in selective autophagy. J Biol Chem., 283(33)，22847-22857, 2008 81. Gotow T, Shiozaki M, Higashi T, Yoshimura K, Shibata M, Kominami E, Uchiyama Y. : Hepatic gap junctions in the hepatocarcinogen-resistant DRH rat. Histochem Cell Biol. In press (2008) 82. Sou YS, Waguri S, Iwata J, Ueno T, Fujimura T, Hara T, Sawada N, Yamada A, Mizushima N, Uchiyama Y, Kominami E, Tanaka K, Komatsu M.: The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice. Mol Biol Cell., 19(11):4762-4775(2008) 83. Haruna K, Suga Y, Muramatsu S, Taneda K, Mizuno Y, Ikeda S, Ueno T, Kominami E, Tanida I, Tanida I, Hanada K.: Differentiation-specific expression and localization of an autophagosomal marker protein (LC3) in human epidermal keratinocytes. J Dermatol Sci., 52(3):213-215(2008) 84. Ichimura Y, Kominami E, Tanaka K, Komatsu M. : Selective turnover of p62/A170/SQSTM1 by autophagy. Autophagy., 4(8);1063-1066(2008) 85. Ebato C, Uchida T, Arakawa M, Komatsu M, Ueno T, Komiya K, Azuma K, Hirose T, Tanaka K, Kominami E, Kawamori R, Fujitani Y, Watada H. : Autophagy is important in islet homeostasis and compensatory increase of beta cell mass in response to high-fat diet. Cell Metab., 8(4):325-332(2008) 86. Gotoh K, Lu Z, Morita M, Shibata M, Koike M, Waguri S, Dono K, Doki Y, Kominami E, Sugioka A, Monden M, Uchiyama Y. : Participation of autophagy in the initiation of graft dysfunction after rat liver transplantation. Autophagy., 5(3): 351-360 (2009) 87. Takahashi K, Ueno T, Tanida I, Minematsu-Ikeguchi N, Murata M, Kominami E. : Characterization of CAA0225, a novel inhibitor specific for cathepsin L, as a probe for autophagic proteolysis. Biol Pharm Bull.,32(3): 475-479 (2009) 88. Shibata M, Yoshimura K, Furuya N, Koike M, Ueno T, Komatsu M, Arai H, Tanaka K, Kominami E, Uchiyama Y. : The MAP1-LC3 conjugation system is involved in lipid droplet formation. Biochem Biophys Res Commun., 382(2): 419-423 (2009) 89. Nakao R, Hirasaka K, Goto J, Ishidoh K, Yamada C, Ohno A, Okumura Y, Nonaka I, Yasutomo K, Baldwin KM, Kominami E, Higashibata A, Nagano K, Tanaka K, Yasui N, Mills EM, Takeda S, Nikawa T. : Ubiquitin ligase Cbl-b is a negative regulator for IGF-1 signaling during muscle atrophy caused by unloading. Mol Cell Biol.29(17): 4798-4811 (2009) 90. Tanida I, Fukasawa M, Ueno T, Kominami E, Wakita T, Hanada K. : Knockdown of autophagy-related gene decreases the production of infectious hepatitis C virus particles. Autophagy.,5(7):937-945 (2009) 91. Kyei GB, Dinkins C, Davis AS, Roberts E, Singh SB, Dong C, Wu L, Kominami E, Ueno T, Yamamoto A, Federico M, Panganiban A, Vergne I, Deretic V. : Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields in macrophages. J Cell Biol.,186(2):255-268 (2009) 92. Kasai M, Tanida I, Ueno T, Kominami E, Seki S, Ikeda T, Mizuochi T. : Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol., 83(11):7278-2785 ( 2009) 英文著書 1. Ueno T,Tanida I,Kominami E: Autophagy and Neuromuscular Diseases. Autophagy, edited by Daniel Klionsky. LANDES BIOSCIENCE, 264-286 (2004) 英文総説 1.Ishidoh K, Kominami E.：Processing and activation of lysosomal proteinases. Biol Chem, 383:1827-1831 (2002) 2.Tanida I, Ueno T, Kominami E.: LC3 conjugation system in mammalian autophagy. The International Journal of Biochemistry Cell Biology, 36: 2503-2518 (2004) 3.Ezaki J, Kominami E.: The intracellular location and function of proteins of neuronal ceroid lipofuscinoses. Brain Pathol., 14: 77-85 (2004) 4.Komatsu M, Ueno T, Waguri S, Uchiyama Y, Kominami E, Tanaka K. Constitutive autophagy: vital role in clearance of unfavorable proteins in neurons. Cell Death Differ. 14(5): 887-894 (2007) 5.Klionsky DJ, Abeliovich H, Agostinis P, Agrawal DK, Aliev G, Askew DS, Baba M, Baehrecke EH, Bahr BA, Ballabio A, Bamber BA, Bassham DC, Bergamini E, Bi X, Biard-Piechaczyk M, Blum JS, Bredesen DE, Brodsky JL, Brumell JH, Brunk UT, Bursch W, Camougrand N, Cebollero E, Cecconi F, Chen Y, Chin LS, Choi A, Chu CT, Chung J, Clarke PG, Clark RS, Clarke SG, ClavｸJ・C, Cleveland JL, Codogno P, Colombo MI, Coto-Montes A, Cregg JM, Cuervo AM, Debnath J, Demarchi F, Dennis PB, Dennis PA, Deretic V, Devenish RJ, Di Sano F, Dice JF, Difiglia M, Dinesh-Kumar S, Distelhorst CW, Djavaheri-Mergny M, Dorsey FC, DrｸJ嚊e W, Dron M, Dunn WA Jr, Duszenko M, Eissa NT, Elazar Z, Esclatine A, Eskelinen EL, FｸJ遠ｸJ糂 L, Finley KD, Fuentes JM, Fueyo J, Fujisaki K, Galliot B, Gao FB, Gewirtz DA, Gibson SB, Gohla A, Goldberg AL, Gonzalez R, GonzｸJ′ez-EstｸJ塩ez C, Gorski S, Gottlieb RA, HｸJｕssinger D, He YW, Heidenreich K, Hill JA, HｸH薮er-Hansen M, Hu X, Huang WP, Iwasaki A, JｸJ・・ttelｸJ・M, Jackson WT, Jiang X, Jin S, Johansen T, Jung JU, Kadowaki M, Kang C, Kelekar A, Kessel DH, Kiel JA, Kim HP, Kimchi A, Kinsella TJ, Kiselyov K, Kitamoto K, Knecht E, Komatsu M, Kominami E, Kondo S, KovｸJ＜s AL, Kroemer G, Kuan CY, Kumar R, Kundu M, Landry J, Laporte M, Le W, Lei HY, Lenardo MJ, Levine B, Lieberman A, Lim KL, Lin FC, Liou W, Liu LF, Lopez-Berestein G, LｸJ剞ez-OtｸJ錘 C, Lu B, Macleod KF, Malorni W, Martinet W, Matsuoka K, Mautner J, Meijer AJ, MelｸJ猿dez A, Michels P, Miotto G, Mistiaen WP, Mizushima N, Mograbi B, Monastyrska I, Moore MN, Moreira PI, Moriyasu Y, Motyl T, MｸJ粮z C, Murphy LO, Naqvi NI, Neufeld TP, Nishino I, Nixon RA, Noda T, NｸJ糅nberg B, Ogawa M, Oleinick NL, Olsen LJ, Ozpolat B, Paglin S, Palmer GE, Papassideri I, Parkes M, Perlmutter DH, Perry G, Piacentini M, Pinkas-Kramarski R, Prescott M, Proikas-Cezanne T, Raben N, Rami A, Reggiori F, Rohrer B, Rubinsztein DC, Ryan KM, Sadoshima J, Sakagami H, Sakai Y, Sandri M, Sasakawa C, Sass M, Schneider C, Seglen PO, Seleverstov O, Settleman J, Shacka JJ, Shapiro IM, Sibirny A, Silva-Zacarin EC, Simon HU, Simone C, Simonsen A, Smith MA, Spanel-Borowski K, Srinivas V, Steeves M, Stenmark H, Stromhaug PE, Subauste CS, Sugimoto S, Sulzer D, Suzuki T, Swanson MS, Tabas I, Takeshita F, Talbot NJ, TallｸJ刄zy Z, Tanaka K, Tanaka K, Tanida I, Taylor GS, Taylor JP, Terman A, Tettamanti G, Thompson CB, Thumm M, Tolkovsky AM, Tooze SA, Truant R, Tumanovska LV, Uchiyama Y, Ueno T, UzcｸJ＃egui NL, van der Klei I, Vaquero EC, Vellai T, Vogel MW, Wang HG, Webster P, Wiley JW, Xi Z, Xiao G, Yahalom J, Yang JM, Yap G, Yin XM, Yoshimori T, Yu L, Yue Z, Yuzaki M, Zabirnyk O, Zheng X, Zhu X, Deter RLGuidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy. 4(2):151-175 (2008) 6.Tanida I, Ueno T, Kominami E.: LC3 and Autophagy. Methods Mol Biol. 445:77-88 (2008)

研究テーマ

タンパク質の分解機構

研究業績（論文）

(2000―2009年）　
英文原著
1. Ichimura, Y., Kirisako, T., Takao, T., Satomi, Y., Shimonishi, Y., Ishihara, N., Mizushima N., Tanida, I., Kominami, E., Ohsumi, M., Noda, T., Ohsumi, Y. ：A ubiquitin-like system mediates protein lipidation . Nature, 408, 488-492 (2000)

2. Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami,E, Ohsumi, Y., Yoshimori, T.: LC3, a mammalian homolog of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J., 19, 5720-5728 (2000)

3. Gotow T, Shibata M, Kanamori S, Tokuno O, Ohsawa Y, Sato N, Isahara K, Yayoi Y, Watanabe T, Leterrier JF, Linden M, Kominami E, Uchiyama Y: Selective localization of Bcl-2 to the inner mitochondrial and smooth endoplasmic reticulum membranes in mammalian cells. Cell Death Differ., 7, 666-674 (2000)

4. Takano-Ohmuro, H., Mukaide, M., Kominami, E., Morioka, K. : Autophagy in embryonic erythroid cells: its role in maturation. Eur. J. Cell Biol., 79: 759-764 (2000)

5. Koike, M., Nakanishi, H., Saftig, P., Ezaki, J., Isahara, K., Ohsawa, Y., Schulz-Schaeffer, W., Watanabe, T., Waguri, S., Kametaka, S., Shibata, M., Yamamoto, K., Kominami, E., Peters, C., von Figura, K., Uchiyama, Y.: Cathepsin D Deficiency Induces Lysosomal Storage with Ceroid Lipofuscin in Mouse CNS Neurons. J. Neurosci., 15, 6898-6906 (2000)

6. Ezaki J, Takeda-Ezaki M, Oda K, and Kominami E.: Characterization of endopeptidase activity of tripeptidyl peptidase-I/ CLN2 protein which is deficient in classical late infantile neuronal ceroid lipofuscinosis Biochem. Biophys.Res.Commun., 268, 904-908 (2000)

7. Ezaki, J., Takeda-Ezaki, M., Kominami, E.: Tripeptidyl peptidase I, the late infantile neuronal ceroid lipofuscinosis gene product, initiates the lysosomal degradation of subunit c of ATP synthase. J. Biochem., 128, 509 – 516 (2000)

8. Suopanki, J., Partanen, S., Ezaki, J., Baumann, M., Kominami, E.TyynelｸJ・ J.: Developmental changes in the expression of NCL-linked proteins. Mol. Genet.Metab., 71, 190-194 (2000)

9. Okazaki, N., Yan, J., Yuasa, S., Ueno, T., Kominami, E., Masuho, Y., Koga, H., Muramatsu, M.: Interaction of Unc-51-like kinase and MAP light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation. Mol. Brain. Res., 85, 1-12 (2000)

10. Iida, T., Nakaseko, C., Ohno, H., Sakuma, M., Ezaki-Takeda, M., Kominami, E., Fujisawa, T., Saito, T.: Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4 containing l ysosomes upon activation of CD4+T cells. J. Immunol., 165, 5062-5068 (2000)

11. Muno, D., Kominami, E., Mizuochi, T.: Generation of both MHC class I- and class II-restricted antigenic peptides from exogenously added ovalbumin in murine phagosomes. FEBS Lett., 478, 178-182 (2000)

12. Kasai, M., Kropshofer, H., Vogt, AB., Kominami, E., Mizuochi, T.: CLIP-derived self peptides bounds to MHC class II molecules of medullary thymic epithelial cells differ from those of cortical thymic epithelial cells In their diversity, length, and C-terminal processing. Eur. J. Immunol., 30, 3542-3551 (2000)

13. Kawada, A., Hara, K., Kominami, E., Tezuka, T., Takahashi, M., Takahara, H.: Precursor of rat epidermal cathepsin L: purification and immunohistochemical localization. J. Dermatol. Sci., 23, 36-45 (2000)

14. Tanida, I., Tanida-Miyake, E., Ueno, T., Kominami, E.:The Human homologue of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates, human Apg12p, GATE-16, GABARAP, and MAP-LC3. J. Biol. Chem., 276, 1701-1706 (2001)

15. Komatsu, M., Tanida, I., Ueno, T., Kiyooka, M., Mariko Ohsumi, M., Ohsumi,Y. Kominami, E.: Dimerization of Apg7p/Cvt2p is essential for E1 activity and interaction with Aut7p/Apg8p and Aut1p/Apg3p. J.Biol. Chem., 276, 9846-9854 (2001)

16. Furuya N, Kanazawa T, Fujimura S, Ueno T, Kominami E, Kadowaki M: Leupeptin-induced appearance of partial fragment of betaine homocysteine methyltransferase during autophagic maturation in rat hepatocytes. J Biochem., 129, 313-320 (2001)

17. Goebel HH, Kominami E, Neuen-Jacob E, Wheeler RB: Morphological studies on CLN2. Europ J Paediatr Neurol. 5 Suppl A:203-207 (2001)

18. Kaneko M, Tomita T, Nakase T, Ohsawa Y, Seki H, Takeuchi E, Takano H, Shi K, Takahi K, Kominami E, Uchiyama Y, Yoshikawa H, Ochi T.: Expression of proteinases and inflammatory cytokines in subchondral bone regions in the destructive joint of rheumatoid arthritis. Rheumatology, 40, 247-255 (2001)

19. Kim BY, Kramer H, Yamamoto A, Kominami E, Kohsaka S, Akazawa C: Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7. J Biol Chem., 276, 29393-29402 (2001)

20. Nakanishi H, Zhang J, Koike M, Nishioku T, Okamoto Y, Kominami E, von Figura K, Peters C, Yamamoto K, Saftig P, Uchiyama Y: Involvement of nitric oxide released from microglia- macrophages in pathological changes of cathepsin D-deficient mice. J Neurosci. 21, 7526-7533 (2001)

21. Shibata M, Kanamori S, Ohsawa Y, Watanabe T, Yayoi Y, Miura M, Kominami E, Uchiyama Y: Prevention of apoptosis of mammalian cells by the CED-3-cleaved form of CED-9. Arch Histol Cytol., 64, 17-28 (2001)

22. Uchida K, Ohmori D, Ueno T, Nishizuka M, Eshita Y, Fukunaga A, Kominami E: Preoviposition activation of cathepsin-like proteinases in degenerating ovarian follicles of the mosquito Culex pipiens pallens. Dev Biol., 237, 68-78 (2001)

23. Waguri S, Kohmura M, Kanamori S, Watanabe T, Ohsawa Y, Koike M, Tomiyama Y, Wakasugi M, Kominami E, Uchiyama Y: Different distribution patterns of the two mannose 6-phosphate receptors in rat liver. J Histochem Cytochem. 49, 1397-1406 (2001)

24. Yayoi Y, Ohsawa Y, Koike M, Zhang G, Kominami E, Uchiyama Y: Specific localization of lysosomal aminopeptidases in type II alveolar epithelial cells of the rat lung. Arch Histol Cytol. 64, 89-97 (2001)

25. Zhang T, Maekawa Y, Sakai T, Nakano Y, Ishii K, Hisaeda H, Kominami E, Katunuma N, Asao T, Himeno K: Splenic cathepsin L is maturated from the proform by interferon-gamma after immunization with exogenous antigens. Biochem Biophys Res Commun. 283, 499-506 (2001)

26. Wheeler RB, Schlie M, Kominami E, Gerhard L, Goebel HH: Neuronal ceroid lipofuscinosis: late infantile or Jansky Bielschowsky type--re-revisited. Acta Neuropathol . 102, 485-488 (2001)

27. Ishidoh K, Kominami E.: Processing and activation of lysosomal proteinases.Biol Chem. 383(12), 1827-1831 (2002)

28. Koike M, Shibata M, Ohsawa Y, Kametaka S, Waguri S, Kominami E, Uchiyama Y.: The expression of tripeptidyl peptidase I in various tissues of rats and mice. Arch Histol Cytol. 65(3), 219-232 (2002)

29. Tanida I, Nishitani T, Nemoto T, Ueno T, Kominami E.: Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing. Biochem Biophys Res Commun. 296(5), 1164-1170 (2002)

30. Yoshida M, Yamashima T, Zhao L, Tsuchiya K, Kohda Y, Tonchev AB, Matsuda M, Kominami E.: Primate neurons show different vulnerability to transient ischemia and response to cathepsin inhibition. Acta Neuropathol , 104(3), 267-272 (2002)

31. Asanuma K, Shirato I, Ishidoh K, Kominami E, Tomino Y.: Selective modulation of the secretion of proteinases and their inhibitors by growth factors in cultured differentiated podocytes. Kidney Int., 62, 822-831 (2002)

32. Yoshikawa M, Uchida S, Ezaki J, Rai T, Hayama A, Kobayashi K, Kida Y, Noda M, Koike M, Uchiyama Y, Marumo F, Kominami E, Sasaki S.: CLC-3 deficiency leads to phenotypes similar to human neuronal ceroid lipofuscinosis. Genes Cells. 7, 597-605 (2002)

33. Kouroku Y, Fujita E, Jimbo A, Kikuchi T, Yamagata T, Momoi MY, Kominami E, Kuida K, Sakamaki K, Yonehara S, Momoi T. : Polyglutamine aggregates stimulate ER stress signals and caspase-12 activation. Hum Mol Genet. 11, 1505-1515, 2002

34. Shibuya S, Wakayama Y, Inoue M, Oniki H, Kominami E. : Changes in the distribution and density of caveolin 3 molecules at the plasma membrane of mdx mouse skeletal muscles: a fracture-label electron microscopic study. Neurosci Lett. 325, 171-174 (2002)

35. Tanida I, Tanida-Miyake E, Nishitani T, Komatsu M, Yamazaki H, Ueno T, Kominami E.: Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs. Biochem Biophys Res Commun., 292, 256-262 (2002)

36. Tanida I, Tanida-Miyake E, Komatsu M, Ueno T, Kominami E. : Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J Biol Chem., 277, 13739-13744 (2002)

37. Tanida I, Komatsu M, Ueno T, Kominami E.: GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3. Biochem Biophys Res Commun., 300, 637-644 (2003)

38. Koike M, Shibata M, Ohsawa Y, Nakanishi H, Koga T, Kametaka S, Waguri S, Momoi T, Kominami E, Peters C, Figura K, Saftig P, Uchiyama Y.: Involvement of two different cell death pathways in retinal atrophy of cathepsin D-deficient mice. Mol Cell Neurosci., 22, 146-161, (2003)

39. Asanuma K, Tanida I, Shirato I, Ueno T, Takahara H, Nishitani T, Kominami E, Tomino Y.: MAP-LC3, a promising autophagosomal marker, is processed during the differentiation and recovery of podocytes from PAN nephrosis. FASEB J., 17, 1165-1167 (2003)

40. Haraguchi CM, Ishido K, Kominami E, Yokota S.: Expression of cathepsin H in differentiating rat spermatids: immunoelectron microscopic study. Histochem Cell Biol., 120, 63-71 (2003)

41. Katoh K, Shibata H, Suzuki H, Nara A, Ishidoh K, Kominami E, Yoshimori T, Maki M.: The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting. J Biol Chem. 278, 39104-39113 (2003)

42. Nemoto T, Tanida I, Tanida-Miyake E, Minematsu-Ikeguchi N, Yokota M, Ohsumi M, Ueno T, Kominami E.: The mouse APG10 homologue, an E2-like enzyme for Apg12p conjugation, facilitates MAP-LC3 modification. J Biol Chem. 278, 39517-39526 (2003)

43. Yamazaki-Sato H, Tanida I, Ueno T, Kominami E.: The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation. FEBS Lett. 551, 71-77 (2003)

44. Yamashima T, Tonchev AB, Tsukada T, Saido TC, Imajoh-Ohmi S, Momoi T, Kominami E.: Sustained calpain activation associated with lysosomal rupture executes necrosis of the postischemic CA1 neurons in primates. Hippocampus., 13, 791-800 (2003)

45. Ezaki J, Takeda-Ezaki M, Koike M, Ohsawa Y, Taka H, Mineki R, Murayama K, Uchiyama Y, Ueno T, Kominami E.: Characterization of Cln3p, the gene product responsible for juvenile neuronal ceroid lipofuscinosis, as a lysosomal integral membrane glycoprotein. J Neurochem. 87, 1296-1308 (2003)

46. Kim BY, Ueda M, Kominami E, Akagawa K, Kohsaka S, Akazawa C.: Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex., Biochem Biophys Res Commun. 311, 577-582 (2003)

47. Tsiakas K, Steinfeld R, Storch S, Ezaki J, Lukacs Z, Kominami E, Kohlschutter A, Ullrich K, Braulke T.: Mutation of the glycosylated asparagine residue 286 in human CLN2 protein results in loss of enzymatic activity. Glycobiology. 14, 1C-5C (2004)

48. Komatsu M, Chiba T, Tatsumi K, Iemura S, Tanida I, Okazaki N, Ueno T, Kominami E, Natsume T, Tanaka K.: A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier. EMBO J., 23, 1977-1986 (2004)

49. Tanida I, Sou YS, Ezaki J, Minematsu-Ikeguchi N, Ueno T, Kominami E.: HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates. J Biol
Chem., 279, 36268-36276 (2004)

50. Reiser J, Oh J, Shirato I, Asanuma K, Hug A, Mundel TM, Honey K, Ishidoh K, Kominami E, Kreidberg JA, Tomino Y, Mundel P.: Podocyte migration during nephrotic syndrome requires a coordinated interplay between cathepsin L and alpha3 integrin. J Biol Chem. 279, 34827-34832., (2004)

51. Kouno T, Mizuguchi M, Tanida I, Ueno T, Kominami E, Kawano K.: 1H, 13C, and 15N resonance assignments of human microtubule-associated protein light chain-3. J Biomol NMR., 29(3):415-416 (2004)

52. Lecaille F, Muno D, Kominami E, Ishidoh K.: Proteinases participating in the processing and activation of prolegumain in primary cultured rat macrophages. Biol Chem., 385, 511-516 (2004)

53. Jager S, Bucci C, Tanida I, Ueno T, Kominami E, Saftig P, Eskelinen EL.: Role for Rab7 in maturation of late autophagic vacuoles. J Cell Sci., 117:4837-4848 2004

54. Tanida I, Ueno T, Kominami E.: Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes. J Biol Chem. 279, 47704-47710 (2004)

55. Komatsu M, Waguri S, Ueno T, Iwata J, Fujimura T, Murata S, Tanida I, Ezaki J, Mizushima N, Ohsumi Y, Kurayama K, Uchiyama Y, Kominami E, Tanaka K, Chiba T : Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol, 169(3), 425-434 (2005)

56. Tanida I, Minematsu-Ikegami N, Ueno T, Kominami E.: Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy, 1, 84-91 (2005)

57. Koike M, Shibata M, Waguri S, Yoshimura K, Tanida I, Kominami E, Gotow T, Peters C, von Figura K, Mizushima N, Saftig P, Uchiyama Y.: Participation of autophagy in storage of lysosomes in neurons from mouse models of neuronal ceroid-lipofuscinoses (Batten disease). Am J Pathol. 167(6):1713-1728 (2005)

58. Kouno T, Mizuguchi M, Tanida I, Ueno T, Kanematsu T, Mori Y, Shinoda H, Hirata M, Kominami E, Kawano K.: Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains. J Biol Chem. 280(26), 24610-24607 (2005)

59. Sou YS, Tanida I, Komatsu M, Ueno T, Kominami E. : Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16. J.Biol Chem. 281(6):3017-3024 (2006)

60. Iwata J, Ezaki J, Komatsu M, Yokota S, Ueno T, Tanida I, Chiba T, Tanaka K, Kominami E.: Excess peroxisomes are degraded by autophagic machinery in mammals. J Biol Chem., 281(7):4035-4041 (2006)

61. Kumanomidou T, Mizushima T, Komatsu M, Suzuki A, Tanida I, Sou YS, Ueno T, Kominami E, Tanaka K, Yamane T. : The crystal structure of human Atg4b, a processing and de-conjugating enzyme for autophagosome-forming modifiers. J Mol Biol. 355(4):612-618. (2006)

62. Sasakawa H, Sakata E, Yamaguchi Y, Komatsu M, Tatsumi K, Kominami E, Tanaka K, Kato K.: Solution structure and dynamics of Ufm1, a ubiquitin-fold modifier 1.　Biochem Biophys Res Commun. 343(1):21-26.（2006）

63. Komatsu M, Waguri S, Chiba T, Murata S, Iwata J, Tanida I, Ueno T, Koike M, Uchiyama Y, Kominami E, Tanaka K.: Loss of autophagy in the central nervous system causes neurodegeneration in mice.　Nature. 441(7095):880-884. （2006）

64. Tanida I, Sou YS, Minematsu-Ikeguchi N, Ueno T, Kominami E.: Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3.　FEBS J. 273(11):2553-2562. （2006）

65. Kouroku Y, Fujita E, Tanida I, Ueno T, Isoai A, Kumagai H, Ogawa S, Kaufman RJ, Kominami E, Momoi T.: ER stress (PERK/eIF2alpha phosphorylation) mediates the polyglutamine-induced LC3 conversion, an essential step for autophagy formation. Cell Death Differ. 14(2):230-239. (2006 )

66. Tanida I, Wakabayashi M, Kanematsu T, Minematsu-Ikeguchi N, Sou YS, Hirata M, Ueno T, Kominami E. : Lysosomal turnover of GABARAP-phospholipid conjugate is activated during differentiation of C2C12 cells to myotubes without inactivation of the mTor kinase-signaling pathway. Autophagy. 2(4):264-271. (2006)

67. Komatsu M, Kominami E, Tanaka K. : Autophagy and neurodegeneration. Autophagy. 2(4):315-317. (2006)

68. Yogosawa S, Kawasaki M, Wakatsuki S, Kominami E, Shiba Y, Nakayama K, Kohsaka S, Akazawa C. : Monoubiquitylation of GGA3 by hVPS18 regulates its ubiquitin-binding ability. Biochem Biophys Res Commun. 350(1):82-90. (2006)

69. Kim BY, Sahara Y, Yamamoto A, Kominami E, Kohsaka S, Akazawa C. : The interaction of mammalian Class C Vps with nSec-1/Munc18-a and syntaxin 1A regulates pre-synaptic release. Biochem Biophys Res Commun. 350(3):691-697. (2006 )

70. Persaud-Sawin DA, Mousallem T, Wang C, Zucker A, Kominami E, Boustany RM. : Neuronal ceroid lipofuscinosis: a common pathway? Pediatr Res. 61(2):146-152. (2007)

71. Yamatake K, Maeda M, Kadowaki T, Takii R, Tsukuba T, Ueno T, Kominami E, Yokota S, Yamamoto K. : Role for gingipains in Porphyromonas gingivalis traffic to phagolysosomes and survival in human aortic endothelial cells. Infect Immun. 75(5):2090-2100. (2007)

72. Mizokami A, Kanematsu T, Ishibashi H, Yamaguchi T, Tanida I, Takenaka K, Nakayama KI, Fukami K, Takenawa T, Kominami E, Moss SJ, Yamamoto T, Nabekura J, Hirata M. : Phospholipase C-related inactive protein is involved in trafficking of gamma2 subunit-containing GABA(A) receptors to the cell surface. J Neurosci. 27(7): 1692-1701 (2007)

73. Komatsu M, Wang QJ, Holstein GR, Friedrich VL Jr, Iwata J, Kominami E, Chait BT, Tanaka K, Yue Z. : Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration. Proc Natl Acad Sci U S A. 104(36):14489-14494. (2007)

74. Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T. : Crystal structure of Ufc1, the Ufm1-conjugating enzyme. Biochem Biophys Res Commun. 362(4):1079-1084. (2007 )

75. Komatsu M, Waguri S, Koike M, Sou YS, Ueno T, Hara T, Mizushima N, Iwata J, Ezaki J, Murata S, Hamazaki J, Nishito Y, Iemura S, Natsume T, Yanagawa T, Uwayama J, Warabi E, Yoshida H, Ishii T, Kobayashi A, Yamamoto M, Yue Z, Uchiyama Y, Kominami E, Tanaka K. : Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell. 131(6):1149-1163.(2007)

76. Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K. : Consideration about negative controls for LC3 and expression vectors for four colored fluorescent protein-LC3 negative controls. Autophagy. 4(1)131-134. (2008)

77. Koike M, Shibata M, Tadakoshi M, Gotoh K, Komatsu M, Waguri S, Kawahara N, Kuida K, Nagata S, Kominami E, Tanaka K, Uchiyama Y.: Inhibition of autophagy prevents hippocampal pyramidal neuron death after hypoxic-ischemic injury. Am J Pathol. 172(2):454-469. (2008)

78. Matsumoto N, Ezaki J, Komatsu M, Takahashi K, Mineki R, Taka H, Kikkawa M, Fujimura T, Takeda-Ezaki M, Ueno T, Tanaka K, Kominami E. : Comprehensive proteomics analysis of autophagy-deficient mouse liver. Biochem Biophys Res Commun. 368(3):643-649. (2008)

79. Ueno T, Sato W, Horie Y, Komatsu M, Tanida I, Yoshida M, Ohshima S, Mak TW, Watanabe S, Kominami E. : Loss of Pten, a tumor suppressor, causes the strong inhibition of autophagy without affecting LC3 lipidation. Autophagy. 4(5):692-700. (2008)

80. Ichimura Y, Kumanomidou T, Sou YS, Mizushima T, Ezaki J, Ueno T, Kominami E, Yamane T, Tanaka K, Komatsu M. : Structural basis for sorting mechanism of p62 in selective autophagy. J Biol Chem., 283(33)，22847-22857, 2008

81. Gotow T, Shiozaki M, Higashi T, Yoshimura K, Shibata M, Kominami E, Uchiyama Y. : Hepatic gap junctions in the hepatocarcinogen-resistant DRH rat. Histochem Cell Biol. In press (2008)

82. Sou YS, Waguri S, Iwata J, Ueno T, Fujimura T, Hara T, Sawada N, Yamada A, Mizushima N, Uchiyama Y, Kominami E, Tanaka K, Komatsu M.: The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice. Mol Biol Cell., 19(11):4762-4775(2008)

83. Haruna K, Suga Y, Muramatsu S, Taneda K, Mizuno Y, Ikeda S, Ueno T, Kominami E, Tanida I, Tanida I, Hanada K.: Differentiation-specific expression and localization of an autophagosomal marker protein (LC3) in human epidermal keratinocytes. J Dermatol Sci., 52(3):213-215(2008)

84. Ichimura Y, Kominami E, Tanaka K, Komatsu M. : Selective turnover of p62/A170/SQSTM1 by autophagy. Autophagy., 4(8);1063-1066(2008)

85. Ebato C, Uchida T, Arakawa M, Komatsu M, Ueno T, Komiya K, Azuma K, Hirose T, Tanaka K, Kominami E, Kawamori R, Fujitani Y, Watada H. : Autophagy is important in islet homeostasis and compensatory increase of beta cell mass in response to high-fat diet. Cell Metab., 8(4):325-332(2008)

86. Gotoh K, Lu Z, Morita M, Shibata M, Koike M, Waguri S, Dono K, Doki Y, Kominami E, Sugioka A, Monden M, Uchiyama Y. : Participation of autophagy in the initiation of graft dysfunction after rat liver transplantation. Autophagy., 5(3): 351-360 (2009)

87. Takahashi K, Ueno T, Tanida I, Minematsu-Ikeguchi N, Murata M, Kominami E. : Characterization of CAA0225, a novel inhibitor specific for cathepsin L, as a probe for autophagic proteolysis. Biol Pharm Bull.,32(3): 475-479 (2009)

88. Shibata M, Yoshimura K, Furuya N, Koike M, Ueno T, Komatsu M, Arai H, Tanaka K, Kominami E, Uchiyama Y. : The MAP1-LC3 conjugation system is involved in lipid droplet formation. Biochem Biophys Res Commun., 382(2): 419-423 (2009)

89. Nakao R, Hirasaka K, Goto J, Ishidoh K, Yamada C, Ohno A, Okumura Y, Nonaka I, Yasutomo K, Baldwin KM, Kominami E, Higashibata A, Nagano K, Tanaka K, Yasui N, Mills EM, Takeda S, Nikawa T. : Ubiquitin ligase Cbl-b is a negative regulator for IGF-1 signaling during muscle atrophy caused by unloading. Mol Cell Biol.29(17): 4798-4811 (2009)

90. Tanida I, Fukasawa M, Ueno T, Kominami E, Wakita T, Hanada K. : Knockdown of autophagy-related gene decreases the production of infectious hepatitis C virus particles. Autophagy.,5(7):937-945 (2009)

91. Kyei GB, Dinkins C, Davis AS, Roberts E, Singh SB, Dong C, Wu L, Kominami E, Ueno T, Yamamoto A, Federico M, Panganiban A, Vergne I, Deretic V. : Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields in macrophages. J Cell Biol.,186(2):255-268 (2009)

92. Kasai M, Tanida I, Ueno T, Kominami E, Seki S, Ikeda T, Mizuochi T. : Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol., 83(11):7278-2785 ( 2009)

英文著書
1. Ueno T,Tanida I,Kominami E: Autophagy and Neuromuscular Diseases. Autophagy, edited by Daniel Klionsky. LANDES BIOSCIENCE, 264-286 (2004)

英文総説
1.Ishidoh K, Kominami E.：Processing and activation of lysosomal proteinases. Biol Chem, 383:1827-1831 (2002)

2.Tanida I, Ueno T, Kominami E.: LC3 conjugation system in mammalian autophagy. The International Journal of Biochemistry Cell Biology, 36: 2503-2518 (2004)

3.Ezaki J, Kominami E.: The intracellular location and function of proteins of neuronal ceroid lipofuscinoses. Brain Pathol., 14: 77-85 (2004)

4.Komatsu M, Ueno T, Waguri S, Uchiyama Y, Kominami E, Tanaka K. Constitutive autophagy: vital role in clearance of unfavorable proteins in neurons. Cell Death Differ. 14(5): 887-894 (2007)

5.Klionsky DJ, Abeliovich H, Agostinis P, Agrawal DK, Aliev G, Askew DS, Baba M, Baehrecke EH, Bahr BA, Ballabio A, Bamber BA, Bassham DC, Bergamini E, Bi X, Biard-Piechaczyk M, Blum JS, Bredesen DE, Brodsky JL, Brumell JH, Brunk UT, Bursch W, Camougrand N, Cebollero E, Cecconi F, Chen Y, Chin LS, Choi A, Chu CT, Chung J, Clarke PG, Clark RS, Clarke SG, ClavｸJ・C, Cleveland JL, Codogno P, Colombo MI, Coto-Montes A, Cregg JM, Cuervo AM, Debnath J, Demarchi F, Dennis PB, Dennis PA, Deretic V, Devenish RJ, Di Sano F, Dice JF, Difiglia M, Dinesh-Kumar S, Distelhorst CW, Djavaheri-Mergny M, Dorsey FC, DrｸJ嚊e W, Dron M, Dunn WA Jr, Duszenko M, Eissa NT, Elazar Z, Esclatine A, Eskelinen EL, FｸJ遠ｸJ糂 L, Finley KD, Fuentes JM, Fueyo J, Fujisaki K, Galliot B, Gao FB, Gewirtz DA, Gibson SB, Gohla A, Goldberg AL, Gonzalez R, GonzｸJ′ez-EstｸJ塩ez C, Gorski S, Gottlieb RA, HｸJｕssinger D, He YW, Heidenreich K, Hill JA, HｸH薮er-Hansen M, Hu X, Huang WP, Iwasaki A, JｸJ・・ttelｸJ・M, Jackson WT, Jiang X, Jin S, Johansen T, Jung JU, Kadowaki M, Kang C, Kelekar A, Kessel DH, Kiel JA, Kim HP, Kimchi A, Kinsella TJ, Kiselyov K, Kitamoto K, Knecht E, Komatsu M, Kominami E, Kondo S, KovｸJ＜s AL, Kroemer G, Kuan CY, Kumar R, Kundu M, Landry J, Laporte M, Le W, Lei HY, Lenardo MJ, Levine B, Lieberman A, Lim KL, Lin FC, Liou W, Liu LF, Lopez-Berestein G, LｸJ剞ez-OtｸJ錘 C, Lu B, Macleod KF, Malorni W, Martinet W, Matsuoka K, Mautner J, Meijer AJ, MelｸJ猿dez A, Michels P, Miotto G, Mistiaen WP, Mizushima N, Mograbi B, Monastyrska I, Moore MN, Moreira PI, Moriyasu Y, Motyl T, MｸJ粮z C, Murphy LO, Naqvi NI, Neufeld TP, Nishino I, Nixon RA, Noda T, NｸJ糅nberg B, Ogawa M, Oleinick NL, Olsen LJ, Ozpolat B, Paglin S, Palmer GE, Papassideri I, Parkes M, Perlmutter DH, Perry G, Piacentini M, Pinkas-Kramarski R, Prescott M, Proikas-Cezanne T, Raben N, Rami A, Reggiori F, Rohrer B, Rubinsztein DC, Ryan KM, Sadoshima J, Sakagami H, Sakai Y, Sandri M, Sasakawa C, Sass M, Schneider C, Seglen PO, Seleverstov O, Settleman J, Shacka JJ, Shapiro IM, Sibirny A, Silva-Zacarin EC, Simon HU, Simone C, Simonsen A, Smith MA, Spanel-Borowski K, Srinivas V, Steeves M, Stenmark H, Stromhaug PE, Subauste CS, Sugimoto S, Sulzer D, Suzuki T, Swanson MS, Tabas I, Takeshita F, Talbot NJ, TallｸJ刄zy Z, Tanaka K, Tanaka K, Tanida I, Taylor GS, Taylor JP, Terman A, Tettamanti G, Thompson CB, Thumm M, Tolkovsky AM, Tooze SA, Truant R, Tumanovska LV, Uchiyama Y, Ueno T, UzcｸJ＃egui NL, van der Klei I, Vaquero EC, Vellai T, Vogel MW, Wang HG, Webster P, Wiley JW, Xi Z, Xiao G, Yahalom J, Yang JM, Yap G, Yin XM, Yoshimori T, Yu L, Yue Z, Yuzaki M, Zabirnyk O, Zheng X, Zhu X, Deter RLGuidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy. 4(2):151-175 (2008)

6.Tanida I, Ueno T, Kominami E.: LC3 and Autophagy. Methods Mol Biol. 445:77-88 (2008)

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木南 英紀（コミナミ エイキ）

木南英紀（コミナミエイキ）