学校法人順天堂　研究者情報データベース:::研究業績詳細:::

HOME　＞　研究業績詳細

研究業績詳細

内山安男（ウチヤマヤスオ）

研究テーマ	オートファジー性神経細胞死の分子細胞生物学的研究
研究業績（論文）	Original Articles Hadano S, Mitsui S, Pan L, Otomo A, Kubo M, Sato K, Ono S, Onodera W, Abe K, Chen X, Koike M, Uchiyama Y, Aoki M, Warabi E, Yamamoto M, Ishii T, Yanagawa T, Shang H, Yoshii F (in press) Functional links between SQSTM1 and ALS2 in the pathogenesis of ALS: Cumulative impact on the protection against mutant SOD1-mediated motor dysfunction in mice. Hum Mol Genet Sunabori T, Koike M, Asari A, Oonuki Y, Uchiyama Y (in press) Suppression of ischemia-induced hippocampal pyramidal neuron death by hyaluronan tetrasaccharide through inhibition of toll-like receptor 2 signaling pathway. Am J Pathol Shibata M, Koike M, Kusumi K, Sato N, Uchiyama U (2016) A specific tripeptidyl substrate for tripeptidyl peptidase activity is effectively hydrolyzed by alanyl aminopeptidase/aminopeptidase N/CD13 in the rat kidney. Arch Histol Cytol 76: 1-8, doi.org/10.1679/aohc.76.1 Yamamoto-Nonaka K, Koike M, Asanuma K, Takagi M, Oliva Trejo JA, Seki T, Hidaka T, Ichimura K, Sakai T, Tada N6 Ueno T, Uchiyama Y, Tomino Y (2016) Cathepsin D in podocytes is important in the pathogenesis of proteinuria and CKD. J Am Soc Nephrol, doi: 10.1681/ASN.2015040366 Xie C, Ginet V, Sun Y, Koike M, Zhou K, Li T, Li H, Li Q, Wang X, Uchiyama Y, Truttmann AC, Kroemer G, Puyal J, Blomgren K, Zhu C (2016) Neuroprotection by selective neuronal deletion of autophagy-related gene 7 in neonatal brain injury. Autophagy 12:410-423, doi: 10.1080/15548627.2015.1132134. Rinchai D, Riyapa D, Buddhisa S, Utispan K, Titball RW, Stevens MP, Stevens JM, Ogawa M, Tanida I, Koike M, Uchiyama Y, Ato M, Lertmemongkolchai G (2015) Macroautophagy is essential for killing of intracellular Burkholderia pseudomallei in human neutrophils. Autophagy 11:748-755. doi: 10.1080/15548627.2015.1040969 Funayama M, Ohe K, Amo T, Furuya N, Yamaguchi J, Saiki S, Li Y, Ogaki K, Ando M, Yoshino H, Tomiyama H, Nishioka K, Hasegawa K, Saiki H, Satake W, Mogushi K, Sasaki R, Kokubo Y, Kuzuhara S, Toda T, Mizuno Y, Uchiyama Y, Ohno K, Hattori N. (2015) CHCHD2 mutations in autosomal dominant late-onset Parkinson's disease: a genome-wide linkage and sequencing study. Lancet Neurol, 14:274-282. doi: 10.1016/S1474-4422(14)70266-2. Epub 2015 Feb 4. PubMed PMID: 25662902 Furuta A, Kikuchi H, Fujita H, Yamada D, Fujiwara Y, Kabuta T, Nishino I, Wada K, Uchiyama Y (2015) Property of Lysosomal Storage Disease associated with Midbrain Pathology in the CNS of LAMP-2-deficient Mice. Am J Pathol 185: 1713-1723, http://dx.doi.org/10.1016/j.ajpath.2015.02.015 Nori S, Okada Y, Nishimura S, Ssaki T, Itakura G, Kobayashi Y, Renault-Mihara F, Shimizu A, Koya I, Yoshida R, Kudoh J, Koike M, Uchiyama Y, Ikeda E, Toyama Y, Nakamura M, Okano H (2015) Long-Term Safety Issues of iPSC-Based Cell Therapy in a Spinal Cord Injury Model: Oncogenic Transformation with Epithelial-Mesenchymal Transition. Stem Cell Rep 4:1-14 Uemura N, Koike M, Ansai K, Kinoshita M, Fujiwara-Ishikawa T, Matsui H, Naruse K, Sakamoto N, Uchiyama Y, Todo T, Takeda S, Yamakado H, Takahashi R (2015) Axonal accumulation of alpha-synuclein in Gaucher disease model of medaka (Oryzias latipes) does not contribute to neurodegeneration. Pros Genetics doi: 10.1371 Nanao T, Koike M, Yamaguchi J, Sasaki M, Uchiyama Y (2015) Cellular localization and tissue distribution of endogenous DFCP1 protein. Biomed Res 36:121-133 Tanida I, Ueno T, Uchiyama Y (2015) A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy. PLoS ONE 9: e110600. doi:10.1371/journal.pone.0110600 Suyama M, Koik M, Asaoka D, Mori H, Oguro M, Ueno T, Nagahara A, Watanabe S, Uchiyama U (2014) Increased immunoreactivity of cathepsins in the rat esophagus under chronic acid reflux esophagitis. J Histochem Cytochem 62:645-660 Sakuraba M, Murata J, Teruyama R, Kamiya K, Yamaguchi J, Okano Y, Uchiyama Y, Ikeda K (2014) Spatotemporal expression of TRPM4 in the mouse cochlea. J Neurosci Res 92:1409-1418 Ueshima　S, Nishida T, Koike M, Matsuda H, Sawa Y, Uchiyama Y (2014) Nirtic oxide-mediated injury of interstitial cells of Cajal and intestinal dysmotility under endotoxemia. Biomed Res 35:251-262 Bartolomé A, Kimura-Koyanagi M, Asahara S, Guillén C, Teruyama K, Inoue H, Shimizu S, Kanno A, García-Aguilar A, Koike M, Uchiyama Y, Benito M, Noda T, Kido Y (2014) Pancreatic β cell failure mediated by mTORC1 hyperactivity and autophagic Pancreatic β cell failure mediated by mTORC1 hyperactivity and autophagic impairment. Diabetes Shigihara N, Fukunaka A, Hara A, Komiya K, Honda A, Uchida T, Abe H, Toyofuku Y, Tamaki M, Ogihara T, Miyatsuka T, Hiddinga HJ, Sakagashira S, Koike M, Uchiyama, Y, Yoshimori T, Eberhardt NL, Fujitani Y, Watada H (2014) Human IAPP-induced pancreastic beta cell toxicity and its regulateon by autophagy. J Clin Inv 124: 3634-3644 DOI: 10.1172/JCI69866 Nakafuku-Fukuda M, Hirata T, Keto Y, Yamano M, Yokoyama T, Uchiyama Y (2014) Inhibitory effect of the selective secretion 5-HT receptor antagonist ramosetron on duodenal acidification-induced gastric hypersensitivity in rats. Eur J Pharmacol 731:88-92 doi: 10.1016/j.ejphar.2014.02.040. Yamanaka T, Tosaki A, Kurosawa M, Matsumoto G, Koike M, Uchiyama Y, Maity SN, Shimogori T, Hattori N, Nukina N (2014) NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin and p62 accumulation and endoplasmic reticulum disorganization. Nat Commun doi:10.1038/ncomms4354 Yokono M, Takasu T, Hayashizaki Y, Mitsuoka K, Kihara R, Muramatsu Y, Miyoshi S, Tahara A, Kurosaki E, Li Q, Tomiyama H, Sasamata M, Shibasaki M, Uchiyama Y (2014) SGLT2 selective inhibitor ipragliflozin reduces body fat mass by increasing fatty acid oxidation in high-fat diet-induced obese rats. Eur J Pharmacol 727-66-74 doi: 10.1016/j.erphar.2014.01.004 Awazawa M, Futami T, Sakada M, Kaneko K, Ohsugi M, Nakaya K, Terai A, Suzuki R, Koike M, Uchiyama Y, Kadowaki T, Ueki K (2014) Deregulation of Pancreas-Specific Oxidoreductin ERO1β in the Pathogenesis of Diabetes Mellitus. Mol Cell Biol 34:1290-1299 doi:10.1128/MCB.01647-13 Kashima J, Shintani-Ishida K, Nakajima M, Maeda H, Unuma K, Uchiyama Y, Yoshida K (2014) Immunocytochemical study of the autophagy marker microtubule-associated protein 1 light chain 3 in normal and steatotic human livers. Hepatol Res, 44:779-787, DOI: 10.1111/hepr.12183 Nonomura K, Yamaguchi Y, Hamachi M, Koike M, Uchiyama Y, Nakazato K, Mochizuki A, Sakaue-Sawano A, Miyawaki A, Yoshida H, Kuida K, Miura M (2013) Local apoptosis modulates early mammalian brain development through the elimination of morphogen-producing cells. Dev Cell 27: 621-634 Abe H, Uchida T, Hara A, Mizukami H, Komiya K, Koike M, Shigihara N, Toyofuku Y, Ogihara T, Uchiyama Y, Yagihashi S, Fujitani Y, Watada H. (2013) Exendin-4 improves beta cell function in autophagy-deficient beta cells. Endicrinology 154: 4512-4524 Sakata K, Ohmuraya M, Araki K, Suzuki C, Ida S, Hashimoto D, Wang J, Uchiyama Y, Baba H, Yamamura K (2013) Generation and analysis of serine protease inhibitor Kazal type 3-Cre driver mice. Exp Animals. Okura H, Kobayashi T, Koike M, Ohsawa M, Zhang D, Arai H, Uchiyama Y, Hino O (2013) Tuberin activates and controls the distribution of Rac1 via association with p62 and ubiquitin through the mTORC1 signaling pathway. Int J Oncol 43: 447-456 Adachi T, Takahara K, Taneo J, Uchiyama Y, Inaba K (2013) Particle size of latex beads dictates IL-1β production mechanism. PLos One 8(7):e68499 Koike M, Tanida I, Nanao N, Tada N, Iwata J, Ueno T, Kominami E, Uchiyama Y (2013) Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons. PLos One 8:e63568 (equally contributed) Matsui, H., Sato, F., Sato, S., Koike, M., Taruno, Y., Saiki, S., Funayama, M., Ito, H., Taniguchi, Y., Uemura, N., Toyoda, A., Sakaki, Y., Takeda, S., Uchiyama, Y., Hattori, N., Takahashi, R. ATP13A2 deficiency induces a decrease in cathepsin D activity, fingerprint-like inclusion body formation, and selective degeneration of dopaminergic neurons. FEBS letters 587(9):1316-25, 2013. (These authors contributed equally to this work) Ohkouchi S, Shibata M, Sasaki M, Koike M, Safig P, Peters C, Nagata S, Uchiyama Y (2013) Biogenesis and proteolytic processing of lysosomal DNase II. PLos One, 8:e59148 Furuta A, Wakabayashi K, Haratake J, Kikuchi H, Kabuta T, Mori F, Tokonami F, Katsumi Y, Tanioka F, Uchiyama Y, Nishino I, Wada K (2013) Lysosomal storage and advanced senescence in the brain of LAMP-2-deficient Danon disease. Acta Neuropathol, 125:459-461 Koike M, Shibata M, Ezaki J, Peters C, Saftig P, Kominami E, Uchiyama Y (2013) Differences in expression patterns of cathepsin C/dipeptidyl peptidase I in normal, pathological and aged mouse central nervous systems. Eur J Neurosci, 37:816-830 Piao X, Komazawa-Sakon S, Nishida T, Koike M, Piao JH, Ehlken H, Kurihara H, Hara M, van Rooijen N, Schütz G, Ohmuraya M, Uchiyama Y, Yagita H, Okumura K, He YW, Nakano H (2012) c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis. Sci Signal doi:10.1126/scsignal. 2003448 Tashiro Y, Urushitani M, Inoue H, Koike M, Uchiyama Y, Komatsu M, Tanaka K, Yamazaki M, Abe M, Misawa H, Sakimura K, Ito H, Takahashi R (2012) Motor Neuron-specific Disruption of Proteasomes, but not Autophagy, Replicates Amyotrophic Lateral Sclerosis. J Biol Chem, 287:42984-42994 Unno T, Wakamori M, Koike M, Uchiyama Y, Ishikawa K, Kubota H, Yoshida T, Sasakawa H, Peters C, Mizusawa H, Watase K (1912) Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6. Proc Natl Acad Sci USA, 109: 17693-17698 Hayakawa N, Shiozaki M, Shibata M, Koike M, Uchiyama Y, Matsuura M, Gotow T (2012) Resveratrol affects undifferentiated and differentiated PC12 cells differently, particularly with respect to possible differences in mitochondrial and autophagic functions. Eur J Neurosci, 92:30-43 Sekine S, Kanamaru Y, Koike M, Nishihara A, Okada M, Kinoshita H, Kamiyama J, Maruyama J, Uchiyama Y, Ishihara N, Takeda K, Ichijo H (2012) Rhomboid protease PARL mediates the mitochondrial membrane potential loss-induced cleavage of PGAM5. J Biol Chem, 287: 34635-34645, PMID: 22915595 Imaizumi Y, Okada Y, Akamatsu W, Koike M, Kuzumaki N, Hayakawa H, Nihira T, Kobayashi T, Ohyama M, Sato S, Takanashi M, Funayama M, Hirayama A, Soga T, Hishiki T, Suematsu M, Yagi T, Ito D, Kosakai A, Hayashi K, Shouji M, Nakanishi A, Suzuki N, Mizushima N, Amagai M, Uchiyama Y, Mochizuki H, Hattori N, Okano H (2012) Mitochondrial dysfunction associated with increased oxidative stress and α-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue. Mol Brain, 5:35 Klionsky D et al. (2012) Guidelines for the use and interpretation of assays for monitoring autophagy. Atuophagy 8:4, 1-100 Kuwahara Y, Oikawa T, Ochiai Y, Roudkenar MH, Fukumoto M, Shimura T, OhtakeY, Ohkubo Y, Mori S, Uchiyama Y, Fukumoto M (2011) Enhancement of autophagy is a potential modality for tumors refractory to radiotherapy. Cell Death Dis, 2:e177 Shiozaki M, Hayakawa N, Shibata M, Koike M, Uchiyama Y, Gotow T (2011) Closer association of mitochondria with lipid droplets in hepatocytes and activation of Kupffer cells in resveratrol-treated senescence-accelerated mice. Histochem Cell Biol, 136:475–489 Nori S, Okada Y, Yasuda A, Tsuji O, Takahashi Y, Kobayashi Y, Fujiyoshi K, Koike M, Uchiyama Y, Ikeda E, Toyama Y, Yamanaka S, Nakamura M, Okano H (2011) Grafted human-induced pluripotent stem-cell-derived neurospheres promote motor functional recovery after spinal cord injury in mice. Proc Natl Acad Sci USA 108: 16825-16830 Uchida Y, Hasegawa J, Chinnapen D, Inoue T, Okazaki S, Kato R, Wakatsuki S, Misaki R, Koike M, Uchiyama Y, Iemura S, Natsume T, Kuwahara R, Nakagawa T, Nishikawa K, Mukai K, Miyoshi E, Taniguchi N, Sheff D, Lencer WI, Taguchi T, Arai H (2011) Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes. Proc Natl Acad Sci USA 108:15846-15851 Koyanagi M, Asahara A, Matsuda T, Hashimoto N, Shigeyama Y, Shibutani Y, Hosooka T, Inoue H, Matsumoto H, Koike M, Uchiyama Y, NodaT, Seino S, Kasuga M, Kido Y (2011) Ablation of TSC2 enhances insulin secretion by increasing the number of mitochondria through activation of mTORC1. PLoS One 6:e23238 Oguro M, Koike M, Ueno T, Asaoka D, Mori H, Nagahara A, Uchiyama Y, Watanabe S (2011) Dissociation and dispersion of claudin-3 from the tight junction could be one of the most sensitive indicators of reflux esophagitis in a rat model of the disease. J Gastroenterol, 46:629-638 Shinohara M, Sato N, Kurinami H, Takeuchi D, Takeda S, Shimamura M, Yamashita T, Uchiyama Y, Rakugi H, Morishita R (2010) Reduction of brain β-amyloid by fluvastatin, a hydroxymethylglutaryl- CoA reductase inhibitor, through increase in degradation of amyloid precursor protein Cterminal fragments (APP-CTFs) and Aβ-clearance. J Biol Chem 285: 22091-22102 Terada K, Yamada J, Hayashi Y, Wu Z, Uchiyama Y, Peters C, Nakanishi H (2010) Involvement of cathepsin B in the processing and secretion of interleukin-1beta in chromogranin AsStimulated microglial. Glia 58: 114-124 Sun L, Wu Z, Baba M, Peters C, Uchiyama Y, Nakanishi H (2010) Cathepsin B-dependent motor neuron death after nerve injury in the adult mouse. Biochem Biophys Res Comm 399: 391-395 Lee J-H, Yu WH, Kumar A, Lee S, Mohan PS, Peterhoff CM, Wolfe DM, Martinez-Vicente M, Massey AC, Sovak G, Uchiyama Y, Westaway D, Cuervo AM, Nixon RA (2010) Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations. Cell 141:1146-1158 Tizon B, Sahoo S, Yu H, Gauthier S, Kumar AR, Mohan P, Figliola M, Pawlik M, Grubb A, Uchiyama Y, Bandyopadhyay U, Cuervo AM, Nixon RA, Levy E. (2010) Induction of autophagy by cystatin C: a mechanism that protects murine primary cortical neurons and neuronal cell lines. PMID: 20541250 Hadano S, Otomo A, Kunita R, Suzuki-Utsunomiya A, Akatsuka A, Koike M, Aoki M, Uchiyama Y, Itoyama Y, Ikeda JE (2010) Loss of ALS2/Alsin Exacerbates Motor Dysfunction in a SOD1H46R-Expressing Mouse ALS Model by Disturbing Endolysosomal Trafficking. PLoS One 5:e9805. Shimada N, Ohno-Matsui K, Iseki S, Koike M, Uchiyama Y, Wang J, Yoshida T, Sato T, Peters C, Mochizuki M, Morita I (2010) Cathepsin L in bone marrow-derived cells is required for retinal and choroidal neovascularization. Am J Pathol 176:2571-2580 Terada K, Yamada J, Hayashi Y, Wu Z, Uchiyama Y, Peters C, Nakanishi H (2010) Involvement of cathepsin B in the processing and secretion of interleukin-1 in chromogranin A-stimulated microglial. 10.1002/glia.20906 Shibata M, Yoshimura K, Tamura H, Ueno T, Nishimura T, Inoue T, Sasaki M, Koike M, Arai H, Kominami E, Uchiyama Y (2010) LC3, a microtubule-associated protein1A/B light chain3, is involved in cytoplasmic lipid droplet formation. Biophys Biochem Res Comm 393:274-279 Yagita Y, Horie K, Yamanaka I, Koinuma S, Shigeyoshi Y, Kawakami K, Takeda J, Uchiyama Y (2010) Chondrogenesis during the ES cell differentiation in vitro. PNAS 107:3846-3851 Tamura H, Shibata M, Koike M, Sasaki M, Uchiyama Y (2010) Atg9A protein, an autophagy-related membrane protein, is localized in the neurons of mouse brains. J Histochem Cytochem 58: 443-453 Maejima Y, Sedbazar U, Suyama S, Kohno D, Onaka T, Takano E, Yoshida N, Koike M, Uchiyama Y, Fujiwara K, Yashiro T, Horvath TL, Dietrich MO, Tanaka S, Dezaki K, Oh-I S, Hashimoto K, Shimizu H, Nakata M, Mori M, Yada T (2009) Nesfatin-1-regulated paraventricular oxytocinergic signaling causes anorexia through a leptin-independent melanocortin pathway. Cell Metabolism 10: 355-365 Matsuda, T., Kido, Y., Nakano-Hashimoto, N., Kaisho, T., Tanaka, T., Asahara, S., Shigeyama, Y., Takeda, A., Inoue, T., Shibutani, Y., Koyanagi, M., Hosooka, T., Matsumoto, M., Inoue, H., Uchida, T., Koike, M., Uchiyama, Y., Akira, S., Kasuga, M. (2009) Ablation of C/EBP beta alleviates ER stress and pancreatic beta cell failure through induction of GRP78. J Clin Invest 120:115-126 Yagita K, Yamanaka I, Koinuma S, Shigeyoshi Y, Uchiyama Y. (2009) Mini Screening of Kinase Inhibitors Affecting Period-length of Mammalian Cellular Circadian Clock. Acta Histochem Cytochem 42:89-93 Sugita S, Horie S, Nakamura O, Maruyama K, Takase H, Usui Y, Takeuchi M, Ishidoh K, Koike M, Uchiyama Y, Peters C, Yamamoto Y, Mochizuki M. (2009) Acquisition of T regulatory function in cathepsin L-inhibited T cells by eye-derived CTLA-2alpha during inflammatory conditions. J Immunol 183:5013-5022 Ohsawa S, Hamada S, Asou H, Kuida K, Uchiyama Y, Yoshida H, Miura M. (2009) Caspase-9 activation revealed by semaphorin 7A cleavage is independent of apoptosis in the aged olfactory bulb. J Neurosci 29:11385-11292. Shibata M, Yoshimura K, Furuya M, Koike M, Ueno T, Komatsu M, Arai H, Tanaka K, Kominami E, Uchiyama Y (2009) The MAP1-LC3 conjugation system is involved in lipid droplet formation. Biophys Biochem Res Comm 382: 419-423 (* equally contributed) Nada S, Hondo A, Kasai A, Koike M, Saito K, Uchiyama Y, Okada M (2009) The novel lipid raft adaptor p18 controls endosome dynamics by anchoring the MEK-ERK pathway to late endosomes. EMBO J 28: 477-489 (* equally contributed) Gotoh K, Lu Z, Morita M, Shibata M, Koike M, Waguri S, DonoK, DokiY, Kominami E, Sugioka A, Monden M, Uchiyama Y (2009) Participation of autophagy in the initiation of graft dysfunction after rat liver transplantation. Autophagy 5:351-360 Garcia M, Rao M, Fujimoto J, Garcia V, Shah S, Crum J, Gotow T, Uchiyama Y, Ellisman MH, Calcutt N, Cleveland D (2009) Phosphorylation of highly conserved NF-M KSP repeats is not required for myelin-dependent radial axonal growth. J Nurosci 29: 1277-1284 Miyamoto K, Shiozaki M, Shibata M, Koike M, Uchiyama Y, Gotow T (2009) Very-high-dose alpha-tocopherol supplementation increases blood pressure and causes possible adverse central nervous system effects in stroke-prone spontaneously hypertensive rats. J Neurosci Res 87: 556-566 Gotow T, Shiozaki M, Higashi T, Yoshimura K, Shibata M, Kominami E, Uchiyama Y (2008) Hepatic gap junctions in the hepatocarcinogen-resistant DRH rat. Histochem Cell Biol 130: 583-594 Fujimoto M, Serada S, Mihara M, Uchiyama Y, Yoshida H, Koike N, Ohsugi Y, Nishikawa T, Ripley B, Kimura A, Kishimoto T, Naka T. (2008) Interleukin-6 blockade suppresses autoimmune arthritis in mice by the inhibition of inflammatory Th17 responses. Arthritis Rheum 58: 3710-3719 Qiao L, Hamamichi S, Caldwell KA, Caldwell GA, Yacoubian TA, Wilson S, Xie Z-L, Speake LD, Parks R, Crabtree D, Liang Q, Crimmins S, Schneider L, Uchiyama Y, Iwatsubo T, Zhou Y, Peng L, Lu YM, Standaert DG, Walls KC, Shacka JJ, Roth KA, Zhang J (2008) Lysosomal enzyme cathepsin D protects against alpha-synuclein aggregation and toxicity. Mol Brain 1:17 Sou YS, Waguri S, Iwata JI, Ueno T, Fujimura T, Hara T, Sawada N, Yamada A, Mizushima N, Uchiyama Y, Kominami E, Tanaka K, Komatsu M (2008) The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice. Mol Biol Cell 19: 4762-4775 Maeda Y, Ide T, Koike M, Uchiyama Y, Kinoshita T (2008) GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. Nat Cell Biol 10: 1135-1145 Mori Y, Koike M, Moriishi E, Kawabata A, Tang H, Oyaizu H, Uchiyama Y, Yamanishi K (2008) Human herpesvirus-6 induces MVB formation and virus egress occurs via an exosomal release pathway. Traffic 9:1728-1742 *equal contributors Saito F, Kuwata H, Oiki E, Koike M, Uchiyama Y, Honda K, Takeda K (2008) Inefficient phagosome maturation in infant macrophages. Biochem Biophys Res Commun 375: 113-118 Yoshioka A, Miyata H, Doki Y, Yamasaki M, Sohma I, Gotoh K, Takiguchi S, Fujiwara Y, Uchiyama Y, Monden M (2008) LC3, an autophagosome marker, is highly expressed in gastrointestinal cancers. Int J Oncol 33:461-468. Oppenheim RW, Blomgren K, Ethell DW, Koike M, Komatsu M, Prevette D, Roth KA, Uchiyama Y, Vinsant S, Zhu C (2008) Developing postmitotic mammalian neurons in vivo lacking Apaf-1 undergo programmed cell death by a caspase-independent, nonapoptotic pathway involving autophagy. J Neurosci 28: 1490-1497 Shiozaki M, Yoshimura K, Shibata M, Koike M, Uchiyama Y, Gotow T (2008) Morphological and biochemical signs of age-related neurodegenerative changes in Klotho mutant mice. Neuroscience 152:924-941 Kiyohara Y, Nishii K, Ukai-Tadenuma M, Ueda H, Uchiyama Y, Yagita K (2008) Detection of a circadian enhancer in the mDbp promoter using prokaryotic transposon vector based strategy. Nucl Acids Res 36 (4): e23 Suzuki C, Takabatake Y, Tanaka H, Koike M, Shibata M, Uchiyama Y, Takahara S, Imai E, Isaka Y (2008) Participation of autophagy in renal ischemia/reperfusion injury. Biochem Biophys Res Comm 368:100-106 Fujibayashi A, Taguchi T, Misaki R, Ohtani M, Dohmae N, Takio K, Yamada M, Gu J, Yamakami M, Fukuda M, Waguri S, Uchiyama Y, Yoshimori T, Sekiguchi K (2008) Human RME-8 is involved in membrane trafficking through early endosomes. Cell Struct Funct 33:35-50 Takatsuka A, Yagi R, Koike M, Oneyama C, Nada S, Schmedt C, Uchiyama Y, Okada M (2008) Ablation of Csk in neural crest lineages causes corneal anomaly by deregulating collagen fibril organization and cell motility. Dev Biol 315:474-488 Hasegawa S, Hamada S, Kumode Y, Esumi S, Katori S, Fukuda E, Uchiyama Y, Hirabayashi T, Mombaerts P, Yagi T (2008) The protocadherin-alpha family is involved in axonal coalescence of olfactory sensory neurons into glomeruli of the olfactory bulb in mouse. Mol Cell Neurosci 38:66-79 Koike M, Shibata S, Tadakoshi T, Gotoh K, Komatsu M, Waguri S, Kawahara N, Kuida K, Nagata S, Kominami E, Tanaka K, Uchiyama Y (2008) Inhibition of autophagy prevents hippocampal pyramidal neuron death after hypoxic-ischemic injury. Am J Pathol 172: 454-469 Klionsky DJ, Abeliovich H, Agostinis P, Agrawal DK, Aliev G, Askew DS, Baba M, Baehrecke EH, Bahr BA, Ballabio A, Bamber BA, Bassham DC, Bergamini E, Bi X, Biard-Piechaczyk M, Blum JS, Bredesen DE, Brodsky JL, Brumell JH, Brunk UT, Bursch W, Camougrand N, Cebollero E, Cecconi F, Chen Y, Chin LS, Choi A, Chu CT, Chung J, Clarke PG, Clark RS, Clarke SG, Clavé C, Cleveland JL, Codogno P, Colombo MI, Coto-Montes A, Cregg JM, Cuervo AM, Debnath J, Demarchi F, Dennis PB, Dennis PA, Deretic V, Devenish RJ, Di Sano F, Dice JF, Difiglia M, Dinesh-Kumar S, Distelhorst CW, Djavaheri-Mergny M, Dorsey FC, Dröge W, Dron M, Dunn WA Jr, Duszenko M, Eissa NT, Elazar Z, Esclatine A, Eskelinen EL, Fésüs L, Finley KD, Fuentes JM, Fueyo J, Fujisaki K, Galliot B, Gao FB, Gewirtz DA, Gibson SB, Gohla A, Goldberg AL, Gonzalez R, González-Estévez C, Gorski S, Gottlieb RA, Häussinger D, He YW, Heidenreich K, Hill JA, Høyer-Hansen M, Hu X, Huang WP, Iwasaki A, Jäättelä M, Jackson WT, Jiang X, Jin S, Johansen T, Jung JU, Kadowaki M, Kang C, Kelekar A, Kessel DH, Kiel JA, Kim HP, Kimchi A, Kinsella TJ, Kiselyov K, Kitamoto K, Knecht E, Komatsu M, Kominami E, Kondo S, Kovács AL, Kroemer G, Kuan CY, Kumar R, Kundu M, Landry J, Laporte M, Le W, Lei HY, Lenardo MJ, Levine B, Lieberman A, Lim KL, Lin FC, Liou W, Liu LF, Lopez-Berestein G, López-Otín C, Lu B, Macleod KF, Malorni W, Martinet W, Matsuoka K, Mautner J, Meijer AJ, Meléndez A, Michels P, Miotto G, Mistiaen WP, Mizushima N, Mograbi B, Monastyrska I, Moore MN, Moreira PI, Moriyasu Y, Motyl T, Münz C, Murphy LO, Naqvi NI, Neufeld TP, Nishino I, Nixon RA, Noda T, Nürnberg B, Ogawa M, Oleinick NL, Olsen LJ, Ozpolat B, Paglin S, Palmer GE, Papassideri I, Parkes M, Perlmutter DH, Perry G, Piacentini M, Pinkas-Kramarski R, Prescott M, Proikas-Cezanne T, Raben N, Rami A, Reggiori F, Rohrer B, Rubinsztein DC, Ryan KM, Sadoshima J, Sakagami H, Sakai Y, Sandri M, Sasakawa C, Sass M, Schneider C, Seglen PO, Seleverstov O, Settleman J, Shacka JJ, Shapiro IM, Sibirny A, Silva-Zacarin EC, Simon HU, Simone C, Simonsen A, Smith MA, Spanel-Borowski K, Srinivas V, Steeves M, Stenmark H, Stromhaug PE, Subauste CS, Sugimoto S, Sulzer D, Suzuki T, Swanson MS, Tabas I, Takeshita F, Talbot NJ, Tallóczy Z, Tanaka K, Tanaka K, Tanida I, Taylor GS, Taylor JP, Terman A, Tettamanti G, Thompson CB, Thumm M, Tolkovsky AM, Tooze SA, Truant R, Tumanovska LV, Uchiyama Y, Ueno T, Uzcátegui NL, van der Klei I, Vaquero EC, Vellai T, Vogel MW, Wang HG, Webster P, Wiley JW, Xi Z, Xiao G, Yahalom J, Yang JM, Yap G, Yin XM, Yoshimori T, Yu L, Yue Z, Yuzaki M, Zabirnyk O, Zheng X, Zhu X, Deter RL (2008) Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 4: 151-175 Iwai K, Koike M, Ohshima S, Miyatake K, Uchiyama Y, Saeki Y, Ishii M (2007) RGS18 acts as a negative regulator of osteoclastogenesis by modulating the acid-sensing OGR1/NFAT signaling pathway. J Bone Miner Res 22:1612-1620 Somboonthum F, Yoshii H, Okamoto S, Koie M, Gomi Y, Uchiyama Y, Takahashi M, Yamanishi K, Mori Y (2007) Generation of a recombinant Oka varicella vaccine expressing mumps virus hemagglutinin-neuraminidase protein as a polyvalent live vaccine. Vaccine 25:8741-8755 Hida T, Ikeda H, Kametaka S, Akazawa C, Kohsaka S, Ebisu S, Uchiyama Y, Waguri S (2007) Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells. Arch Histol Cytol 70:303-312 Tulone C, Uchiyama Y, Novelli Y, Grosvenor N, Saftig P, Chain BM (2007) Haematopoietic development and immunological function in the absence of cathepsin D. BMC Immunology 2007, 8:22 doi:10.1186/1471-2172-8-22 Miyanishi M, Tada K, Koike M, Uchiyama, Y, Kitamura T, Nagata S (2007) Identification of Tim-4 as a phosphatidylserine receptor. Nature 450: 435-439 Komatsu M, Waguri S, Koike M, Sou1 Y, Ueno T, Hara H, Mizushima N, Iwata J, Ezaki J, MurataS, Hamazaki J, Nishito Y, Iemura S, Natsume T, Yanagawa T, Uwayama J, Warabi E, Yoshida H, Ishii T, Kobayashi A, Yamamoto M, Yue Z, Uchiyama Y, Kominami E, Tanaka T (2007) Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131: 1149-1163 Yamasaki R, Zhang J, Koshiishi I, Suniarti DFS, Wu Z, Peters C, Schwake M, Uchiyama Y, Kira J, Saftig P, Utsumi H, Nakanishi H (2007) Involvement of lysosomal storage-induced p38 MAP kinase activation in the overproduction of nitric oxide by microglia in cathepsin D-deficient mice. Mol Cell Neurosci 35: 573-584 Yamanaka I, Koinuma S, Shigeyoshi Y, Uchiyama Y, Yagita K (2007) Presence of robust circadian clock oscillation under constitutive over-expression of mCry1 in rat-1 fibroblasts. FEBS Lett 581:4098-4102 Nakahara, M., Nagasaka, A., Koike, M., Uchida, K., Kawane, K., Uchiyama, Y., Nagata, S. (2007) Degradation of nuclear DNA by DNase II-like acid DNase in cortical fiber cells of mouse eye lens. FEBS J 274: 3055-3064 Komatsu, M, Ueno, T, Waguri, S, Uchiyama, Y, Kominami, E, Tanaka, K (2007) Constitutive autophagy: vital role in clearance of unfavorable proteins in neurons. Cell Death Diff 14:887-894 Shacka JJ, Klocke BJ, Young C, Shibata M, Olney JW, Uchiyama Y, Saftig P, Roth KA (2007) Cathepsin D Deficiency Induces Persistent Neurodegeneration in the Absence of Bax-Dependent Apoptosis. J Neurosci, 27: 2081-2090 Yagi R, Waguri S, Sumikawa S, Nada S, Oneyama C, Itami S, Schmedt C, Uchiyama Y, Okada M (2007) C-terminal Src kinase controls development and maintenance of mouse squamous epithelia. EMBO J 26: 1234-1244 Shacka JJ, Lu J, Xie ZL, Uchiyama Y, Roth KA, Zhang J. (2007) Kainic acid induces early and transient autophagic stress in mouse hippocampus. Neurosci Lett. 414: 57-60 Zhu, J-H, Horbinski, C., Guo, F., Watkins, S., Uchiyama, Y., Chu, C.T. (2006) Reguration of autophagy by extracellular signal regulated protein kinases during 1-methyl-4-phenylpyridinium injury. Am J Pathol 170:75-86 Waguri, S., Tomiyama, Y., Hida, T., Sakai, N., Taniike, M., Wakasugi, M., Ebisu, S., Uchiyama, Y. (2006) The luminal domain participates in the endosomal trafficking of the cation-independent mannose 6-phosphate receptor. Exp. Cell Res. 312: 4090-4107 Yamada S, Isojima Y, Kanamori S, Waguri S, Uchiyama Y, Nagai K. (2006) Uncoupling protein 2 negatively regulates neurite extensions in PC12h cells. Neurosci Lett. 410(2):110-114 Yae K, Keng VW, Koike M, Yusa K, Kouno M, Uno Y, Kondoh G, Gotow T, Uchiyama Y, Horie K, Takeda J. (2006) Sleeping beauty transposon-based phenotypic analysis of mice: lack of Arpc3 results in defective trophoblast outgrowth. Mol Cell Biol. 26: 6185-6196 Ishii M, Iwai K, Koike M, Ohshima S, Kudo-Tanaka E, Ishii T, Mima T, Katada Y, Miyatake K, Uchiyama Y, Saeki Y. (2006) RANKL-induced expression of tetraspanin CD9 in lipid raft membrane microdomain is essential for cell fusion during osteoclastogenesis. J Bone Miner Res. 21: 965-976 Yoshimura K, Shibata , Koike M, Gotoh K, Fukaya M, Watanabe M, Uchiyama Y (2006) Effects of RNA interference of Atg4B on the limited proteolysis of LC3 in PC12 cells and expression of Atg4B in various rat tissues. Autophagy 2: 200-208 Komatsu M, Waguri S, Chiba T, Murata S, Iwata J, Ueno Koike M, Uchiyama Y, Kominami E, Tanaka K (2006) Loss of autophagy in the central nervous system causes neurodegeneration. Nature 441: 880-884 Shimamura M, Sato N, Waguri S, Uchiyama Y, Hayashi T, Iida H, Nakamura T, Ogihara T, Kaneda Y, Morishita R (2006) Gene transfer of HGF gene improves learning and memory in the chronic stage of cerebral infarction. Hypertension 47: 742-751 Zhu C, Xu F, Wang X, Shibata M, Uchiyama Y, Blomgren K, Hagberg H (2006) Different apoptotic mechanisms are activated in male and female brains after neonatal hypoxia-ischaemia. J Neurochem, 96: 1016–1027 Shacka J, Klocke BJ, Shibata M, Uchiyama Y, Datta G, Schmidt RE, Roth KA (2006) Bafilomycin A1 Inhibits Chloroquine-Induced Death of Cerebellar Granule Neurons. Mol Pharacol 69: 1125-1136 Sasaki T, Gotow T, Shiozaki M, Sakaue F, Saito T, Julien JP, Uchiyama Y, Hisanaga S. (2006) Aggregate formation and phosphorylation of neurofilament-L Pro22 Charcot-Marie-Tooth disease mutants. Hum Mol Genet. 15:943-952 Hye-Won Shin, Hiromi Kobayashi, Masashi Kitamura, Satoshi Waguri, Tatsuo Suganuma, Yasuo Uchiyama, and Kazuhisa Nakayama (2005) Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking. J Cell Sci 118: 4039-4048 Koike M, Shibata M, Waguri S, Yoshimura K, Tanida I, Kominami E, Gotow G, Peters C, Figura Kv, Mizushima N, Saftig P and Uchiyama Y (2005) Participation of autophagy in storage of lysosomes in neurons from mouse models of neuronal ceroid-lipofuscinoses (Batten disease). Am J Pathol 167:1713-1728 Oonuki Y, Yoshida Y, Uchiyama Y, Asari A (2005) Application of fluorophore-assisted carbohydrate electrophoresis to analysis of disaccharides and oligosaccharides derived from glycosaminoglycans. Anal Biochem 343: 212-222 Yu WH, Cuervo AM, Kumar A, Peterhoff CM, Schmidt SD, Lee JH, Mohan PS, Mercken M, Farmery MR, Tjernberg LO, Jiang Y, Duff K, Uchiyama Y, J Näslund, Mathews PM, Cataldo AM, Nixon RA (2005) Macroautophagy－a novel amyloid-b (Ab) peptide-generating athway activated in alzheimer’s disease. J Cell Biol 171: 87-98 Takemoto M, Koike M, Mori Y, Yonemoto S, Sasamoto Y, Kondo K, Uchiyama Y, Yamanishi K (2005) Human herpesvirus 6 ORF14 protein and cellular p53 interact with each other and are contained in the virus. J Virol 79: 13037-13046 Yoshida H, Kawane K, Koike M, Mori Y, Uchiyama Y, Nagata, S (2005) Phosphatidylserine-dependent engulfment by macrophages of nuclei from erythroid precursor cells. Nature 434: 754-758 Shimizu, T., Hayashi, Y., Yamada, J., Zhang, J., Yamasaki, J., Ukai, K., Koike, M., Mine, K., Figura, K., Peters, C., Saftig, P., Fukuda, T., Uchiyama, Y., Nakanishi, H. (2005) Proteolytic Degradation of Glutamate Decarboxylase Mediates Disinhibition of Hippocampal CA3 Pyramidal Cells in Cathepsin D-deficient Mice. J Neurochem 94:680-690 Komatsu M, Waguri S, Ueno T, Iwata J, Murata S, Tanida I, Ezaki J, Mizushima N, Ohsumi Y, Uchiyama Y, Kominami E, Tanaka K, Chiba T (2005) Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol 169: 425–434 Lu Z, Dono K, Gotoh K, Shibata M, Koike M, Marubashi S, Miyamoto A, Takeda Y, Nagano H, Umeshita K, Uchiyama Y, Monden M (2005) Participation of autophagy in the degeneration process of rat hepatocytes after transplantation following prolonged cold preservation. Arch Histol Cytol 68: 71-80 Zhu C, Wang X, Xu F, Bahr BA, Shibata M, Uchiyama Y, Hagberg H, Blomgren K (2005) The influence of age on apoptotic and other mechanisms of cell death after cerebral hypoxia-ischemia. Cell Death Differ 12:162-176 Hashimoto N, Kido Y, Uchida T, Matsuda T, Suzuki K, Inoue H, Matsumoto M, Ogawa W, Maeda S, Uchiyama Y, Ohno S, Noda T, Kasuga M (2005) PKCλ in pancreatic β-cells mediates glucose-induced insulin secretion via the regulation of gene expression. J Clin Inv 115:138-145 Yu WH, Kumar A, Peterhoff C, Shapiro Kulnane L, Uchiyama Y, Lamb BT, Cuervo AM, Nixon RA (2004) Autophagic vacuoles are enriched in amyloid precursor protein-secretase activities: implications for beta-amyloid peptide over-production and localization in Alzheimer's disease. Int J Biochem Cell Biol 36: 2531-2540. Tomiyama Y, Waguri S, Kanamori S, Kametaka S, Wakasugi M, Shibata M, Ebisu S, Uchiyama Y (2004) Early-phase redistribution of the cation-independent mannose 6-phosphate receptor by U18666A treatment in HeLa cells. Cell Tissue Res 317: 253-264 Hanayama R, Tanaka M, Miyasaka K, Aozasa K, Koike M, Uchiyama Y, Nagata S (2004) Autoimmune Disease and Impaired Uptake of Apoptotic Cells in MFG-E8-Deficient Mice. Science 304:1147-1150 Mori Y, Akkapaiboon P, Yonemoto S, Koike M, Takemoto M, Sadaoka T, Sasamoto Y, Konishi S, Uchiyama Y, Yamanishi K (2004) Discovery of a second form of tripartite complex containing gH-gL of human herpesvirus 6 and observations on CD46. J Virol 78:4609-4616 Yoshizaki T, Inaji M, Kouike H, Shimazaki T, Sawamoto K, Ando K, Date I, Kobayashi K, Suhara T, Uchiyama Y, Okano H. (2004) Isolation and transplantation of dopaminergic neurons generated from mouse embryonic stem cells. Neurosci Lett. 363:33-37 Shiba, T.,Shiba, Kametaka, S., Kawasaki, M., Shibata, M., Waguri, S., Uchiyama, Y., Wakatsuki, S. (2004) Insights into the phosphoregulation of beta-secretase sorting signal by the VHS domain of GGA1. Traffic 5:437-448 Shimamura, M., Sato, N., Ohshima, K., Aoki, M., Kurinami, H., Waguri, S., Uchiyama, Y., Ogiwara, T., Kaneda, Y., Morishita, R. (2004) Novel therapeutic strategy to treat brain ischemia overexpression of hepatocyte growth factor gene reduced ischemic injury without cerebral edema in rat model. Circulation 109: 424-431 Ezaki J, Takeda-Ezaki M, Koike M, Ohsawa Y, Taka H, Mineki R, Murayama K, Uchiyama Y, Ueno T, Kominami E (2003) Characterization of Cln3p, the gene product responsible for juvenile neuronal ceroid lipofuscinosis, as a lysosomal integral membrane glycoprotein. J Neurochem. 87:1296-1308 Mala V. Rao, Jabbar Campbell, Aidong Yuan, Asok Kumar, Takahiro Gotow, Yasuo Uchiyama, Ralph A. Nixon (2003) The Neurofilament middle molecular weight subunit carboxyl terminal tail domains are essential for the radial growth and cytoskeletal architecture of the axon but not for the regulation of rate of neurofilament transport. J. Cell Biol. 163: 1021-1031 Garcia, M.L., C. S. Lobsiger, S. B. Shah, T. J. Deerinck, J. Crum, D. Young, C. M. Ward, T. O. Crawford, T. Gotow, Y. Uchiyama, M. H. Ellisman, N. A. Calcutt, D. W. Cleveland (2003) NF-M is an essential target for the myelin-directed "outside in" signaling cascade that mediates radial axonal growth. J Cell Biol. 163: 1011-1020 Nishimoto, S., Kawane, K., Watanabe-Fukunaga, R., Fukuyama, H., Ohsawa, Y., Uchiyama, Y., Hashida, N., Ohguro, N., Tano, Y., Morimoto, T., Fukuda, Y., Nagata, S. (2003) Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens. Nature 424: 1071-1074. Wakasugi M., Waguri S., Kametaka S., Tomiyama, Y., Kanamori, S., Shiba, Y., Nakayama, K., Uchiyama, Y. (2003) Predominant expression of the short form of GGA3 in human cell lines and tissues. Biochem Biophys Res Commun 306: 687-692 Yabuta, N., Kajimura, N., Mayanagi, K., Sato, M., Gotow, T., Uchiyama, Y., Ishimi, Y., Nojima, H. (2003) Mammalian Mcm2/4/6/7 complex forms a toroidal structure. Genes Cells 8:413-421 Kametaka S, Shibata M, Moroe K, Kanamori S, Ohsawa Y, Waguri S, Sims PJ, Emoto K, Umeda M, Uchiyama Y. (2003) Identification of phospholipid scramblase 1 as a novel interacting molecule with b -secretase (b-site amyloid precursor protein (APP) processing enzyme (BACE)). J Biol Chem. 278:15239-15245 Koike, M., Shibata, M., Ohsawa, Y., Nakanishi, H., Koga, T., Kametaka, S., Waguri, S., Momoi, T., Kominami, E., Peters, C., Figura, K.von, Saftig, S., Uchiyama, Y. (2003) Involvement of two different cell death pathways in retinal atrophy of cathepsin D-deficient mice. Mol. Cell. Neurosci. 14:142-155 Zhang, G., Ohsawa, Y., Kametaka, S., Shibata, M., Waguri, S., Uchiyama, Y. (2003) Regulation of FLRG expression in rat primary astroglial cells and injured brain tissue by transforming growth factor-beta1 (TGF-beta1). J. Neurosci. Res. 72: 33-45 Kawane, K., Fukuyama, H., Yoshida, H., Nagase H., Ohsawa, Y., Uchiyama, Y., Nagaya, S. (2003) Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation. Nat Immunol 4:138-144 Sakai, Y., Hosaka, M., Hira, Y., Harumi, T., Ohsawa, Y., Wang, H., Takeuchi, T., Uchiyama, Y., Watanabe, T. (2003) Immunocytochemical localization of secretogranin III in the anterior lobe of male rat pituitary glands. J. Histochem. Cytochem. 51: 227-238 Sakakibara, S., Nakamura, Y., Yoshida, T., Shibata, S., Koike, M., Takano, H., Ueda, S., Uchiyama, Y., Noda, T., Okano, H. (2003) RNA-binding protein Musashi family: Roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation. Proc. Natl. Acad. Sci. USA 99:15194-15199 Waguri, S., Dewitte, F., Borgne, R., Rouille, Y., Uchiyama, Y., Dubremetz, J.F., Hoflack, B. (2003) Visualization of TGN to endosomes trafficking through fluorescently labeled MPR and AP-1 in living cells. Mol. Biol. Cell 14: 142-155 Takahashi Y, Jeong SY, Ogata K, Goto J, Hashida H, Isahara K, Uchiyama Y, Kanazawa I. (2003) Human skeletal muscle calcium channel alpha1S is expressed in the basal ganglia: distinctive expression pattern among L-type Ca(2+) channels. Neurosci Res. 45:129-137 Nishimura, F., Naruishi, H., Naruishi, K., Yamada, T., Sasaki, J., Peters, C., Uchiyama, Y., Murayama, Y. (2002) Cathepsin L, a key molecule in the pathogenesis of drug-induced and I-cell disease-mediated gingival hyperplasia: a study with cathepsin L deficient mice. Am. J. Pathol. 161: 2047-2052 Hosaka, M., Watanabe, T., Sakai, Y., Uchiyama, Y., Takeuchi, T. (2002) Identification of a Chromogranin A Domain that Mediates Binding to Secretogranin III and Targeting to Secretory Granules in Pituitary Cells and Pancreatic b-Cells. Mol. Biol. Cell 13:3389-3399 Rao, M.V., Garcia, M.L., Miyazaki, Y., Gotow, T., Yuan, A., Mattina, S., Ward, C.M., Calcutt, N.A., Uchiyama, Y., Nixon, R.A., Cleaveland, D.W. (2002) Gene Replacement in mice reveals that the heavily phosphorylated tail of neurofilament heavy subunit (NF-H) does not affect axonal caliber or the transit of cargoes in slow axonal transport. J. Cell Biol. 158:681-93 Ogawa Y, Sawamoto K, Miyata T, Miyao S, Watanabe M, Nakamura M, Bregman BS, Koike M, Uchiyama Y, Toyama Y, Okano H. (2002) Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats. J Neurosci Res 69:925-933 Suzuki, H., Yanaka, A., Shibahara, T., Matsui, H., Nakahara, A., Tanaka, N., Muto, H., Momoi, T., Y. Uchiyama (2002) Ammonia-induced apoptosis is accelerated at higher pH in gastric surface mucous cells. Am. J. Physiol. Gastrointest. Liver Physiol. 283:G986-995 Koike, M., Shibata, M., Ohsawa, Y., Waguri, S., Kominami, E., Uchiyama, Y. (2002) Expression of tripeptidyl peptidase I in various tissues of rats and mice. Arch. Histol. Cytol. 65:219-232 Shinotsuka, C., Waguri, S., Wakasugi, S., Uchiyama, Y., Nakayama, K. (2002) Dominant negative mutant of BIG2, an ARF-guanin nucleotide exchange factor, specifically affects membrane trafficking from the trans-Golgi network through inhibiting membrane association of AP-1 and GGA coat proteins. Biochem. Biophys. Rep. Comm. 294: 254-260 Xu H, Ito T, Tawada A, Maeda H, Yamanokuchi H, Isahara K, Yoshida K, Uchiyama Y, Asari A (2002) Effect of hyaluronan oligosaccharides on the expression of heat shock protein 72. J Biol Chem 277:17308-17314 Yoshikawa, M., Uchida, S., Ezaki, J., Rai, T., Hayama, A., Kobayashi, K., Kida, Y., Noda, M., Koike, M., Uchiyama, Y., Marumo, F., Kominami, E., Sasaki, S. (2002) CLC-3 deficiency leads to phenotypes similar to human neuronal ceroid lipofuscinosis. Genes to Cells 7: 597-605 Shibata, M., Koike, M., Waguri, S., Zhang, G., Koga, T., Uchiyama, Y. (2002) Cathepsin D is specifically inhibited by deoxyribonucleic acids. FEBS Letters 517:281-284 Ariga K, Yonenobu K, Nakase T, Kaneko M, Okuda S, Uchiyama Y, Yoshikawa H. (2001) Localization of cathepsins D, K, and L in degenerated human intervertebral discs. Spine 26:2666-2672. Kaneko, M., Tomita, T., Nakase, T., Ohsawa, Y., Seki, H., Takeuchi, E., Takano, H., Shi, K., Takahi, K., Kominami, E., Uchiyama, Y., Yoshikawa, H., Ochi, T. (2001) Expression of proteinases and inflammatory cytokines in subchondral bone regions in the destructive joint of rheumatoid arthritis. Rheumatology (Oxford) 40: 247-255 Hirabayashi, M., Inoue, K., Tanaka, K., Nakadate, K., Ohsawa, Y., Kamei. Y., Popiel, A.H., Sinohara, A., Iwamatsu, A., Kimura, Y., Uchiyama, Y., Hori, S., Kakizuka, A. (2001) VCP/p97 in abnormal protein aggregates, cytoplasmic vacuoles, and cell death, phenotypes relevant to neurodegeneration. Cell Death Differ 8: 977-984 Takahashi, K., Takeuchi, J., Takahashi, T., Miyauchi, S., Horie, K., Uchiyama, Y. (2001) Effects of sodium hyaluronate on epithelial healing of the vesical mucosa and vesical fibrosis in rabbits with acetic acid induced cystitis. J. Urology 166: 710-713 Waguri, S., Kohmura, M., Kanamori, S., Watanabe, T., Ohsawa, Y., Koike, M., Tomiyama, Y., Wakasugi, M., Kominami, E., Uchiyama, Y. (2001) Different distribution patterns of the two mannose 6-phosphate receptors in rat liver. J. Histochem. Cytochem. 49: 1397-1405 Nakanishi, H., Zang, J., Koike, M., Nishioku, T., Okamoto, Y., Kominami, E., Figura, K.v., Peters, C., Yamamoto, K., Saftig, P., Uchiyama, Y. (2001) Involvement of nitric oxide release from microglia/macrophages in pathological changes of cathepsin D-deficient mice. J. Neurosci. 21: 7526-7533 Yaginuma, H., Shiraiwa, N., Shimada, T., Nishiyama, K., Hong, J., Wang, S., Momoi, M., Uchiyama, Y., Oppenheim, R. (2001) Caspase activity is involved in, but is dispensable for, early motoneuron death in the chick embryo cervical spinal cord. Mol. Cell. Neurosci. 18: 168-182 Kawane, K., Fukuyama, H., Kondoh, G., Takeda, J., Ohsawa, Y., Uchiyama, Y., Nagata, S. (2001) Requirment of Dnase II for definitive erythropoiesis in the mouse fetal liver. Science 292: 1437-1596 Kawazoe, Y., Naka, T., Fujimoto, M., Kohzaki, H., Morita, Y., Narazaki, M., Okumura, K., Saitoh, H., Nakagawa, R., Uchiyama, Y., Akira, S., Kishimoto, T. (2001) SSI-1/SOCS1 inhibits insulin signal transduction pathway through modulating IRS-1 phosphorylation. J Exp. Med. 193:263-269 Shibata, M., Kanamori, S., Ohsawa, Y., Watanabe, T., Yayoi, Y., Miura, M., Uchiyama, Y. (2001) Prevention of Apoptosis of Mammalian Cells by the CED-3-Cleaved Form of CED-9. Arch. Histol. Cytol. 64: 17-28 Yayoi, Y., Ohsawa, Y., Koike, M., Zhang, G., Kominami, E., Uchiyama , Y. (2001) Specific Localization of Lysosomal Aminopeptidases in Type II Alveolar Epithelial Cells of Rat Lung. Arch. Histol. Cytol. 64: 89-97 IF: 1.503 Sapp, E., Kegel, K.B., Aronin, N., Hashikawa, T., Uchiyama, Y., Tohyama, K., Bhide, P.G., Vonsattel, J.P., DiFiglia, M (2001) Early and progressive accumulation of reactive microglia in the Huntington Disease brain. J. Neuropathol. Exp. Neurol. 60: 161-172 McIlroy, D., Tanaka, M., Sakahira, H., Fukuyama, H., Suzuki, M., Yamamura, K., Ohsawa, Y., Uchiyama Y., Nagata, S. (2000) Apoptotic DNA fragmentation by phagocytosis. Gene Dev 14:549-558 (88) Chung, K.C., Park, J.H., Kim, C.H., Lee, H.W., Sato, N., Uchiyama, Y., Ahn, Y.S. (2000) Novel biphasic effect of pyrrolidine dithiocarbamate on neuronal cell viability is mediated by the differential regulation of intracellular zinc and copper ion levels, NF-kB, and MAP kinases. J. Neurosci. Res. 59: 117-125. (41) Jeong, S., Goto, J., Hashida, H., Suzuki, T., Ogata, K., Masuda, N., Hirai, M., Isahara, K., Uchiyama, Y., Kanazawa, I. (2000) Identification of a novel human voltage-gated sodium channel a subunit gene, SCN12A. Biochem. Biophys. Res. Comm. 267: 262-270 (21) Ogata, K., Jeong, S., Murakami, H., Hashida, H., Suzuki, T., Masuda, N., Hirai, M., Isahara, K., Uchiyama, Y., Goto, J., Kanazawa, I. (2000) Cloning and expression of study of the mouse tetrodotoxin-resistant voltage-gated sodium channel a subunit NaT/Scn11a. Biochem. Biophys. Res. Comm. 267: 271-277. (5) Gotow, T., Shibata, M., Kanamori, S., Tokuno, O., Ohsawa, Y., Sato, N., Isahara, K., Yayoi, Y., Watanabe, T., Leterrier, J.F., Linden, M., Kominami, E., Uchiyama, Y. (2000) Selective localization of Bcl-2 to the inner mitochondrial and smooth endoplasmic reticul¨membranes in mammalian cells. Cell Death Diff. 7: 666-674 (24) Haque, M., Chen, J., Ueda, K., Mori, Y., Nakano, K., Hirata, Y., Kanamori, S., Uchiyama, Y., Inagi, R., Okuno, T., Yamanishi, K. (2000) Identification and analysis of the K5 gene of Kaposi's sarcoma-associated herpesvirus. J. Virol. 74: 2867-75 (40) Koike, M., Nakanishi, H., Saftig, P., Ezaki, J., Isahara, K., Ohsawa, Y., Schulz-Schaeffer, W., Watanabe, T., Waguri, W., Kametaka, S., Shibata, M., Yamamoto, K., Kominami, E., Peters, C., Figura, K., Uchiyama, Y. (2000) Cathepsin D deficiency induces lysosomal storage with ceroid lipofuscin in mouse CNS neurons. J. Neurosc. 20:6898-6906 (80) Fujita, E., Urase, K., Egashira, J., Miho, Y., Isahara, K., Uchiyama, Y., Isoai, A., Kumagai, H., Kuida, K., Motoyama, N., Momoi, T. (2000) Detection of caspase-9 activation in the cell death of Bcl-x-deficient mouse embryo nervous system by cleavage sites-directed antisera. Brain Res. Dev. Brain Res. 122: 135-147 (18) Sato, M., Gotow, T., You, Z., Komamura-Kohno, Y., Uchiyama, Y., Yabuta, N., Nojima, H., Ishimi, Y. (2000) Electron microscopic observation and single-stranded DNA binding activity of the Mcm4,6,7 complex. J. Mol. Biol. 300: 421-431 (48) Matsumura, H., Ohsawa, Y., Kawahara, A., Uchiyama, Y., Nagata, S. (2000) Necrotic pathway in Fas receptor signaling. J. Cell Biol. 151:1247-1255 (99) Nakase, T., Takeuchi, E., Sugimoto, K., Kaneko, M., Tomoi, T., Myoui, A., Uchiyama, Y., Ochi, T., Yoshikawa, H. (2000) Involvement of multinucleated giant cells synthetizing cathepsin K in calcified tendinitis of the rotator cuff tendons. Rheumatology 39: 1074-1077 Nakase, T. Kaneko, M., Tomita, T., Myoui, A., Ariga, K., Sugimoto, K., Uchiyama, Y., Ochi, T., Yoshikawa, H. (2000) Immunohistochemical detection of cathepsin D, K, and L in the process of endochondral ossification in the human. Histoch. Cell Biol. 114: 21-27 (26)

研究テーマ

オートファジー性神経細胞死の分子細胞生物学的研究

研究業績（論文）

Original Articles

Hadano S, Mitsui S, Pan L, Otomo A, Kubo M, Sato K, Ono S, Onodera W, Abe K, Chen X, Koike M, Uchiyama Y, Aoki M, Warabi E, Yamamoto M, Ishii T, Yanagawa T, Shang H, Yoshii F (in press) Functional links between SQSTM1 and ALS2 in the pathogenesis of ALS: Cumulative impact on the protection against mutant SOD1-mediated motor dysfunction in mice. Hum Mol Genet

Sunabori T, Koike M, Asari A, Oonuki Y, Uchiyama Y (in press) Suppression of ischemia-induced hippocampal pyramidal neuron death by hyaluronan tetrasaccharide through inhibition of toll-like receptor 2 signaling pathway. Am J Pathol

Shibata M, Koike M, Kusumi K, Sato N, Uchiyama U (2016) A specific tripeptidyl substrate for tripeptidyl peptidase activity is effectively hydrolyzed by alanyl aminopeptidase/aminopeptidase N/CD13 in the rat kidney. Arch Histol Cytol 76: 1-8, doi.org/10.1679/aohc.76.1

Yamamoto-Nonaka K, Koike M, Asanuma K, Takagi M, Oliva Trejo JA, Seki T, Hidaka T, Ichimura K, Sakai T, Tada N6 Ueno T, Uchiyama Y, Tomino Y (2016) Cathepsin D in podocytes is important in the pathogenesis of proteinuria and CKD. J Am Soc Nephrol, doi: 10.1681/ASN.2015040366

Xie C, Ginet V, Sun Y, Koike M, Zhou K, Li T, Li H, Li Q, Wang X, Uchiyama Y, Truttmann AC, Kroemer G, Puyal J, Blomgren K, Zhu C (2016) Neuroprotection by selective neuronal deletion of autophagy-related gene 7 in neonatal brain injury. Autophagy 12:410-423, doi: 10.1080/15548627.2015.1132134.

Rinchai D, Riyapa D, Buddhisa S, Utispan K, Titball RW, Stevens MP, Stevens JM, Ogawa M, Tanida I, Koike M, Uchiyama Y, Ato M, Lertmemongkolchai G (2015) Macroautophagy is essential for killing of intracellular Burkholderia pseudomallei in human neutrophils. Autophagy 11:748-755. doi: 10.1080/15548627.2015.1040969

Funayama M, Ohe K, Amo T, Furuya N, Yamaguchi J, Saiki S, Li Y, Ogaki K, Ando M, Yoshino H, Tomiyama H, Nishioka K, Hasegawa K, Saiki H, Satake W, Mogushi K, Sasaki R, Kokubo Y, Kuzuhara S, Toda T, Mizuno Y, Uchiyama Y, Ohno K, Hattori N. (2015) CHCHD2 mutations in autosomal dominant late-onset Parkinson's disease: a genome-wide linkage and sequencing study. Lancet Neurol, 14:274-282. doi: 10.1016/S1474-4422(14)70266-2. Epub 2015 Feb 4. PubMed PMID: 25662902

Furuta A, Kikuchi H, Fujita H, Yamada D, Fujiwara Y, Kabuta T, Nishino I, Wada K, Uchiyama Y (2015) Property of Lysosomal Storage Disease associated with Midbrain Pathology in the CNS of LAMP-2-deficient Mice. Am J Pathol 185: 1713-1723, http://dx.doi.org/10.1016/j.ajpath.2015.02.015

Nori S, Okada Y, Nishimura S, Ssaki T, Itakura G, Kobayashi Y, Renault-Mihara F, Shimizu A, Koya I, Yoshida R, Kudoh J, Koike M, Uchiyama Y, Ikeda E, Toyama Y, Nakamura M, Okano H (2015) Long-Term Safety Issues of iPSC-Based Cell Therapy in a Spinal Cord Injury Model: Oncogenic Transformation with Epithelial-Mesenchymal Transition. Stem Cell Rep 4:1-14

Uemura N, Koike M, Ansai K, Kinoshita M, Fujiwara-Ishikawa T, Matsui H, Naruse K, Sakamoto N, Uchiyama Y, Todo T, Takeda S, Yamakado H, Takahashi R (2015) Axonal accumulation of alpha-synuclein in Gaucher disease model of medaka (Oryzias latipes) does not contribute to neurodegeneration. Pros Genetics doi: 10.1371

Nanao T, Koike M, Yamaguchi J, Sasaki M, Uchiyama Y (2015) Cellular localization and tissue distribution of endogenous DFCP1 protein. Biomed Res 36:121-133

Tanida I, Ueno T, Uchiyama Y (2015) A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy. PLoS ONE 9: e110600. doi:10.1371/journal.pone.0110600

Suyama M, Koik M, Asaoka D, Mori H, Oguro M, Ueno T, Nagahara A, Watanabe S, Uchiyama U (2014) Increased immunoreactivity of cathepsins in the rat esophagus under chronic acid reflux esophagitis. J Histochem Cytochem 62:645-660

Sakuraba M, Murata J, Teruyama R, Kamiya K, Yamaguchi J, Okano Y, Uchiyama Y, Ikeda K (2014) Spatotemporal expression of TRPM4 in the mouse cochlea. J Neurosci Res 92:1409-1418

Ueshima　S, Nishida T, Koike M, Matsuda H, Sawa Y, Uchiyama Y (2014) Nirtic oxide-mediated injury of interstitial cells of Cajal and intestinal dysmotility under endotoxemia. Biomed Res 35:251-262

Bartolomé A, Kimura-Koyanagi M, Asahara S, Guillén C, Teruyama K, Inoue H, Shimizu S, Kanno A, García-Aguilar A, Koike M, Uchiyama Y, Benito M, Noda T, Kido Y (2014) Pancreatic β cell failure mediated by mTORC1 hyperactivity and autophagic Pancreatic β cell failure mediated by mTORC1 hyperactivity and autophagic impairment. Diabetes

Shigihara N, Fukunaka A, Hara A, Komiya K, Honda A, Uchida T, Abe H, Toyofuku Y, Tamaki M, Ogihara T, Miyatsuka T, Hiddinga HJ, Sakagashira S, Koike M, Uchiyama, Y, Yoshimori T, Eberhardt NL, Fujitani Y, Watada H (2014) Human IAPP-induced pancreastic beta cell toxicity and its regulateon by autophagy. J Clin Inv 124: 3634-3644 DOI: 10.1172/JCI69866

Nakafuku-Fukuda M, Hirata T, Keto Y, Yamano M, Yokoyama T, Uchiyama Y (2014) Inhibitory effect of the selective secretion 5-HT receptor antagonist ramosetron on duodenal acidification-induced gastric hypersensitivity in rats. Eur J Pharmacol 731:88-92 doi: 10.1016/j.ejphar.2014.02.040.

Yamanaka T, Tosaki A, Kurosawa M, Matsumoto G, Koike M, Uchiyama Y, Maity SN, Shimogori T, Hattori N, Nukina N (2014) NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin and p62 accumulation and endoplasmic reticulum disorganization. Nat Commun doi:10.1038/ncomms4354

Yokono M, Takasu T, Hayashizaki Y, Mitsuoka K, Kihara R, Muramatsu Y, Miyoshi S, Tahara A, Kurosaki E, Li Q, Tomiyama H, Sasamata M, Shibasaki M, Uchiyama Y (2014) SGLT2 selective inhibitor ipragliflozin reduces body fat mass by increasing fatty acid oxidation in high-fat diet-induced obese rats. Eur J Pharmacol 727-66-74 doi: 10.1016/j.erphar.2014.01.004

Awazawa M, Futami T, Sakada M, Kaneko K, Ohsugi M, Nakaya K, Terai A, Suzuki R, Koike M, Uchiyama Y, Kadowaki T, Ueki K (2014) Deregulation of Pancreas-Specific Oxidoreductin ERO1β in the Pathogenesis of Diabetes Mellitus. Mol Cell Biol 34:1290-1299 doi:10.1128/MCB.01647-13

Kashima J, Shintani-Ishida K, Nakajima M, Maeda H, Unuma K, Uchiyama Y, Yoshida K (2014) Immunocytochemical study of the autophagy marker microtubule-associated protein 1 light chain 3 in normal and steatotic human livers. Hepatol Res, 44:779-787, DOI: 10.1111/hepr.12183

Nonomura K, Yamaguchi Y, Hamachi M, Koike M, Uchiyama Y, Nakazato K, Mochizuki A, Sakaue-Sawano A, Miyawaki A, Yoshida H, Kuida K, Miura M (2013) Local apoptosis modulates early mammalian brain development through the elimination of morphogen-producing cells. Dev Cell 27: 621-634

Abe H, Uchida T, Hara A, Mizukami H, Komiya K, Koike M, Shigihara N, Toyofuku Y, Ogihara T, Uchiyama Y, Yagihashi S, Fujitani Y, Watada H. (2013) Exendin-4 improves beta cell function in autophagy-deficient beta cells. Endicrinology 154: 4512-4524

Sakata K, Ohmuraya M, Araki K, Suzuki C, Ida S, Hashimoto D, Wang J, Uchiyama Y, Baba H, Yamamura K (2013) Generation and analysis of serine protease inhibitor Kazal type 3-Cre driver mice. Exp Animals.

Okura H, Kobayashi T, Koike M, Ohsawa M, Zhang D, Arai H, Uchiyama Y, Hino O (2013) Tuberin activates and controls the distribution of Rac1 via association with p62 and ubiquitin through the mTORC1 signaling pathway. Int J Oncol 43: 447-456

Adachi T, Takahara K, Taneo J, Uchiyama Y, Inaba K (2013) Particle size of latex beads dictates IL-1β production mechanism. PLos One 8(7):e68499

Koike M, Tanida I, Nanao N, Tada N, Iwata J, Ueno T, Kominami E, Uchiyama Y (2013) Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons. PLos One 8:e63568 (*equally contributed)

*Matsui, H., *Sato, F., Sato, S., Koike, M., Taruno, Y., Saiki, S., Funayama, M., Ito, H., Taniguchi, Y., Uemura, N., Toyoda, A., Sakaki, Y., Takeda, S., Uchiyama, Y., Hattori, N., Takahashi, R. ATP13A2 deficiency induces a decrease in cathepsin D activity, fingerprint-like inclusion body formation, and selective degeneration of dopaminergic neurons. FEBS letters 587(9):1316-25, 2013. (*These authors contributed equally to this work)

Ohkouchi S, Shibata M, Sasaki M, Koike M, Safig P, Peters C, Nagata S, Uchiyama Y (2013) Biogenesis and proteolytic processing of lysosomal DNase II. PLos One, 8:e59148

Furuta A, Wakabayashi K, Haratake J, Kikuchi H, Kabuta T, Mori F, Tokonami F, Katsumi Y, Tanioka F, Uchiyama Y, Nishino I, Wada K (2013) Lysosomal storage and advanced senescence in the brain of LAMP-2-deficient Danon disease. Acta Neuropathol, 125:459-461

Koike M, Shibata M, Ezaki J, Peters C, Saftig P, Kominami E, Uchiyama Y (2013) Differences in expression patterns of cathepsin C/dipeptidyl peptidase I in normal, pathological and aged mouse central nervous systems. Eur J Neurosci, 37:816-830

Piao X, Komazawa-Sakon S, Nishida T, Koike M, Piao JH, Ehlken H, Kurihara H, Hara M, van Rooijen N, Schütz G, Ohmuraya M, Uchiyama Y, Yagita H, Okumura K, He YW, Nakano H (2012) c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis. Sci Signal doi:10.1126/scsignal. 2003448

Tashiro Y, Urushitani M, Inoue H, Koike M, Uchiyama Y, Komatsu M, Tanaka K, Yamazaki M, Abe M, Misawa H, Sakimura K, Ito H, Takahashi R (2012) Motor Neuron-specific Disruption of Proteasomes, but not Autophagy, Replicates Amyotrophic Lateral Sclerosis. J Biol Chem, 287:42984-42994

Unno T, Wakamori M, Koike M, Uchiyama Y, Ishikawa K, Kubota H, Yoshida T, Sasakawa H, Peters C, Mizusawa H, Watase K (1912) Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6. Proc Natl Acad Sci USA, 109: 17693-17698

Hayakawa N, Shiozaki M, Shibata M, Koike M, Uchiyama Y, Matsuura M, Gotow T (2012) Resveratrol affects undifferentiated and differentiated PC12 cells differently, particularly with respect to possible differences in mitochondrial and autophagic functions. Eur J Neurosci, 92:30-43

Sekine S, Kanamaru Y, Koike M, Nishihara A, Okada M, Kinoshita H, Kamiyama J, Maruyama J, Uchiyama Y, Ishihara N, Takeda K, Ichijo H (2012) Rhomboid protease PARL mediates the mitochondrial membrane potential loss-induced cleavage of PGAM5. J Biol Chem, 287: 34635-34645, PMID: 22915595

Imaizumi Y, Okada Y, Akamatsu W, Koike M, Kuzumaki N, Hayakawa H, Nihira T, Kobayashi T, Ohyama M, Sato S, Takanashi M, Funayama M, Hirayama A, Soga T, Hishiki T, Suematsu M, Yagi T, Ito D, Kosakai A, Hayashi K, Shouji M, Nakanishi A, Suzuki N, Mizushima N, Amagai M, Uchiyama Y, Mochizuki H, Hattori N, Okano H (2012) Mitochondrial dysfunction associated with increased oxidative stress and α-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue. Mol Brain, 5:35

Klionsky D et al. (2012) Guidelines for the use and interpretation of assays for monitoring autophagy. Atuophagy 8:4, 1-100

Kuwahara Y, Oikawa T, Ochiai Y, Roudkenar MH, Fukumoto M, Shimura T, OhtakeY, Ohkubo Y, Mori S, Uchiyama Y, Fukumoto M (2011) Enhancement of autophagy is a potential modality for tumors refractory to radiotherapy. Cell Death Dis, 2:e177

Shiozaki M, Hayakawa N, Shibata M, Koike M, Uchiyama Y, Gotow T (2011) Closer association of mitochondria with lipid droplets in hepatocytes and activation of Kupffer cells in resveratrol-treated senescence-accelerated mice. Histochem Cell Biol, 136:475–489

Nori S, Okada Y, Yasuda A, Tsuji O, Takahashi Y, Kobayashi Y, Fujiyoshi K, Koike M, Uchiyama Y, Ikeda E, Toyama Y, Yamanaka S, Nakamura M, Okano H (2011) Grafted human-induced pluripotent stem-cell-derived neurospheres promote motor functional recovery after spinal cord injury in mice. Proc Natl Acad Sci USA 108: 16825-16830

Uchida Y, Hasegawa J, Chinnapen D, Inoue T, Okazaki S, Kato R, Wakatsuki S, Misaki R, Koike M, Uchiyama Y, Iemura S, Natsume T, Kuwahara R, Nakagawa T, Nishikawa K, Mukai K, Miyoshi E, Taniguchi N, Sheff D, Lencer WI, Taguchi T, Arai H (2011) Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes. Proc Natl Acad Sci USA 108:15846-15851

Koyanagi M, Asahara A, Matsuda T, Hashimoto N, Shigeyama Y, Shibutani Y, Hosooka T, Inoue H, Matsumoto H, Koike M, Uchiyama Y, NodaT, Seino S, Kasuga M, Kido Y (2011) Ablation of TSC2 enhances insulin secretion by increasing the number of mitochondria through activation of mTORC1. PLoS One 6:e23238

Oguro M, Koike M, Ueno T, Asaoka D, Mori H, Nagahara A, Uchiyama Y, Watanabe S (2011) Dissociation and dispersion of claudin-3 from the tight junction could be one of the most sensitive indicators of reflux esophagitis in a rat model of the disease. J Gastroenterol, 46:629-638

Shinohara M, Sato N, Kurinami H, Takeuchi D, Takeda S, Shimamura M, Yamashita T, Uchiyama Y, Rakugi H, Morishita R (2010) Reduction of brain β-amyloid by fluvastatin, a hydroxymethylglutaryl- CoA reductase inhibitor, through increase in degradation of amyloid precursor protein Cterminal fragments (APP-CTFs) and Aβ-clearance. J Biol Chem 285: 22091-22102

Terada K, Yamada J, Hayashi Y, Wu Z, Uchiyama Y, Peters C, Nakanishi H (2010) Involvement of cathepsin B in the processing and secretion of interleukin-1beta in chromogranin AsStimulated microglial. Glia 58: 114-124

Sun L, Wu Z, Baba M, Peters C, Uchiyama Y, Nakanishi H (2010) Cathepsin B-dependent motor neuron death after nerve injury in the adult mouse. Biochem Biophys Res Comm 399: 391-395

Lee J-H, Yu WH, Kumar A, Lee S, Mohan PS, Peterhoff CM, Wolfe DM, Martinez-Vicente M, Massey AC, Sovak G, Uchiyama Y, Westaway D, Cuervo AM, Nixon RA (2010) Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations. Cell 141:1146-1158

Tizon B, Sahoo S, Yu H, Gauthier S, Kumar AR, Mohan P, Figliola M, Pawlik M, Grubb A, Uchiyama Y, Bandyopadhyay U, Cuervo AM, Nixon RA, Levy E. (2010) Induction of autophagy by cystatin C: a mechanism that protects murine primary cortical neurons and neuronal cell lines. PMID: 20541250

Hadano S, Otomo A, Kunita R, Suzuki-Utsunomiya A, Akatsuka A, Koike M, Aoki M, Uchiyama Y, Itoyama Y, Ikeda JE (2010) Loss of ALS2/Alsin Exacerbates Motor Dysfunction in a SOD1H46R-Expressing Mouse ALS Model by Disturbing Endolysosomal Trafficking. PLoS One 5:e9805.

Shimada N, Ohno-Matsui K, Iseki S, Koike M, Uchiyama Y, Wang J, Yoshida T, Sato T, Peters C, Mochizuki M, Morita I (2010) Cathepsin L in bone marrow-derived cells is required for retinal and choroidal neovascularization. Am J Pathol 176:2571-2580

Terada K, Yamada J, Hayashi Y, Wu Z, Uchiyama Y, Peters C, Nakanishi H (2010) Involvement of cathepsin B in the processing and secretion of interleukin-1 in chromogranin A-stimulated microglial. 10.1002/glia.20906

Shibata M, Yoshimura K, Tamura H, Ueno T, Nishimura T, Inoue T, Sasaki M, Koike M, Arai H, Kominami E, Uchiyama Y (2010) LC3, a microtubule-associated protein1A/B light chain3, is involved in cytoplasmic lipid droplet formation. Biophys Biochem Res Comm 393:274-279

Yagita Y, Horie K, Yamanaka I, Koinuma S, Shigeyoshi Y, Kawakami K, Takeda J, Uchiyama Y (2010) Chondrogenesis during the ES cell differentiation in vitro. PNAS 107:3846-3851

Tamura H, Shibata M, Koike M, Sasaki M, Uchiyama Y (2010) Atg9A protein, an autophagy-related membrane protein, is localized in the neurons of mouse brains. J Histochem Cytochem 58: 443-453

Maejima Y, Sedbazar U, Suyama S, Kohno D, Onaka T, Takano E, Yoshida N, Koike M, Uchiyama Y, Fujiwara K, Yashiro T, Horvath TL, Dietrich MO, Tanaka S, Dezaki K, Oh-I S, Hashimoto K, Shimizu H, Nakata M, Mori M, Yada T (2009) Nesfatin-1-regulated paraventricular oxytocinergic signaling causes anorexia through a leptin-independent melanocortin pathway. Cell Metabolism 10: 355-365

Matsuda, T., Kido, Y., Nakano-Hashimoto, N., Kaisho, T., Tanaka, T., Asahara, S., Shigeyama, Y., Takeda, A., Inoue, T., Shibutani, Y., Koyanagi, M., Hosooka, T., Matsumoto, M., Inoue, H., Uchida, T., Koike, M., Uchiyama, Y., Akira, S., Kasuga, M. (2009) Ablation of C/EBP beta alleviates ER stress and pancreatic beta cell failure through induction of GRP78. J Clin Invest 120:115-126

Yagita K, Yamanaka I, Koinuma S, Shigeyoshi Y, Uchiyama Y. (2009) Mini Screening of Kinase Inhibitors Affecting Period-length of Mammalian Cellular Circadian Clock. Acta Histochem Cytochem 42:89-93

Sugita S, Horie S, Nakamura O, Maruyama K, Takase H, Usui Y, Takeuchi M, Ishidoh K, Koike M, Uchiyama Y, Peters C, Yamamoto Y, Mochizuki M. (2009) Acquisition of T regulatory function in cathepsin L-inhibited T cells by eye-derived CTLA-2alpha during inflammatory conditions. J Immunol 183:5013-5022

Ohsawa S, Hamada S, Asou H, Kuida K, Uchiyama Y, Yoshida H, Miura M. (2009) Caspase-9 activation revealed by semaphorin 7A cleavage is independent of apoptosis in the aged olfactory bulb. J Neurosci 29:11385-11292.

Shibata M*, Yoshimura K*, Furuya M, Koike M, Ueno T, Komatsu M, Arai H, Tanaka K, Kominami E, Uchiyama Y (2009) The MAP1-LC3 conjugation system is involved in lipid droplet formation. Biophys Biochem Res Comm 382: 419-423 (* equally contributed)

Nada S*, Hondo A*, Kasai A*, Koike M*, Saito K, Uchiyama Y, Okada M (2009) The novel lipid raft adaptor p18 controls endosome dynamics by anchoring the MEK-ERK pathway to late endosomes. EMBO J 28: 477-489 (* equally contributed)

Gotoh K, Lu Z, Morita M, Shibata M, Koike M, Waguri S, DonoK, DokiY, Kominami E, Sugioka A, Monden M, Uchiyama Y (2009) Participation of autophagy in the initiation of graft dysfunction after rat liver transplantation. Autophagy 5:351-360

Garcia M, Rao M, Fujimoto J, Garcia V, Shah S, Crum J, Gotow T, Uchiyama Y, Ellisman MH, Calcutt N, Cleveland D (2009) Phosphorylation of highly conserved NF-M KSP repeats is not required for myelin-dependent radial axonal growth. J Nurosci 29: 1277-1284

Miyamoto K, Shiozaki M, Shibata M, Koike M, Uchiyama Y, Gotow T (2009) Very-high-dose alpha-tocopherol supplementation increases blood pressure and causes possible adverse central nervous system effects in stroke-prone spontaneously hypertensive rats. J Neurosci Res 87: 556-566

Gotow T, Shiozaki M, Higashi T, Yoshimura K, Shibata M, Kominami E, Uchiyama Y (2008) Hepatic gap junctions in the hepatocarcinogen-resistant DRH rat. Histochem Cell Biol 130: 583-594

Fujimoto M, Serada S, Mihara M, Uchiyama Y, Yoshida H, Koike N, Ohsugi Y, Nishikawa T, Ripley B, Kimura A, Kishimoto T, Naka T. (2008) Interleukin-6 blockade suppresses autoimmune arthritis in mice by the inhibition of inflammatory Th17 responses. Arthritis Rheum 58: 3710-3719

Qiao L, Hamamichi S, Caldwell KA, Caldwell GA, Yacoubian TA, Wilson S, Xie Z-L, Speake LD, Parks R, Crabtree D, Liang Q, Crimmins S, Schneider L, Uchiyama Y, Iwatsubo T, Zhou Y, Peng L, Lu YM, Standaert DG, Walls KC, Shacka JJ, Roth KA, Zhang J (2008) Lysosomal enzyme cathepsin D protects against alpha-synuclein aggregation and toxicity. Mol Brain 1:17

Sou YS, Waguri S, Iwata JI, Ueno T, Fujimura T, Hara T, Sawada N, Yamada A, Mizushima N, Uchiyama Y, Kominami E, Tanaka K, Komatsu M (2008) The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice. Mol Biol Cell 19: 4762-4775

Maeda Y, Ide T, Koike M, Uchiyama Y, Kinoshita T (2008) GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. Nat Cell Biol 10: 1135-1145

Mori Y*, Koike M*, Moriishi E, Kawabata A, Tang H, Oyaizu H, Uchiyama Y, Yamanishi K (2008) Human herpesvirus-6 induces MVB formation and virus egress occurs via an exosomal release pathway. Traffic 9:1728-1742 *equal contributors

Saito F, Kuwata H, Oiki E, Koike M, Uchiyama Y, Honda K, Takeda K (2008) Inefficient phagosome maturation in infant macrophages. Biochem Biophys Res Commun 375: 113-118

Yoshioka A, Miyata H, Doki Y, Yamasaki M, Sohma I, Gotoh K, Takiguchi S, Fujiwara Y, Uchiyama Y, Monden M (2008) LC3, an autophagosome marker, is highly expressed in gastrointestinal cancers. Int J Oncol 33:461-468.

Oppenheim RW, Blomgren K, Ethell DW, Koike M, Komatsu M, Prevette D, Roth KA, Uchiyama Y, Vinsant S, Zhu C (2008) Developing postmitotic mammalian neurons in vivo lacking Apaf-1 undergo programmed cell death by a caspase-independent, nonapoptotic pathway involving autophagy. J Neurosci 28: 1490-1497

Shiozaki M, Yoshimura K, Shibata M, Koike M, Uchiyama Y, Gotow T (2008) Morphological and biochemical signs of age-related neurodegenerative changes in Klotho mutant mice. Neuroscience 152:924-941

Kiyohara Y, Nishii K, Ukai-Tadenuma M, Ueda H, Uchiyama Y, Yagita K (2008) Detection of a circadian enhancer in the mDbp promoter using prokaryotic transposon vector based strategy. Nucl Acids Res 36 (4): e23

Suzuki C, Takabatake Y, Tanaka H, Koike M, Shibata M, Uchiyama Y, Takahara S, Imai E, Isaka Y (2008) Participation of autophagy in renal ischemia/reperfusion injury. Biochem Biophys Res Comm 368:100-106

Fujibayashi A, Taguchi T, Misaki R, Ohtani M, Dohmae N, Takio K, Yamada M, Gu J, Yamakami M, Fukuda M, Waguri S, Uchiyama Y, Yoshimori T, Sekiguchi K (2008) Human RME-8 is involved in membrane trafficking through early endosomes. Cell Struct Funct 33:35-50

Takatsuka A, Yagi R, Koike M, Oneyama C, Nada S, Schmedt C, Uchiyama Y, Okada M (2008) Ablation of Csk in neural crest lineages causes corneal anomaly by deregulating collagen fibril organization and cell motility. Dev Biol 315:474-488

Hasegawa S, Hamada S, Kumode Y, Esumi S, Katori S, Fukuda E, Uchiyama Y, Hirabayashi T, Mombaerts P, Yagi T (2008) The protocadherin-alpha family is involved in axonal coalescence of olfactory sensory neurons into glomeruli of the olfactory bulb in mouse. Mol Cell Neurosci 38:66-79

Koike M, Shibata S, Tadakoshi T, Gotoh K, Komatsu M, Waguri S, Kawahara N, Kuida K, Nagata S, Kominami E, Tanaka K, Uchiyama Y (2008) Inhibition of autophagy prevents hippocampal pyramidal neuron death after hypoxic-ischemic injury. Am J Pathol 172: 454-469

Klionsky DJ, Abeliovich H, Agostinis P, Agrawal DK, Aliev G, Askew DS, Baba M, Baehrecke EH, Bahr BA, Ballabio A, Bamber BA, Bassham DC, Bergamini E, Bi X, Biard-Piechaczyk M, Blum JS, Bredesen DE, Brodsky JL, Brumell JH, Brunk UT, Bursch W, Camougrand N, Cebollero E, Cecconi F, Chen Y, Chin LS, Choi A, Chu CT, Chung J, Clarke PG, Clark RS, Clarke SG, Clavé C, Cleveland JL, Codogno P, Colombo MI, Coto-Montes A, Cregg JM, Cuervo AM, Debnath J, Demarchi F, Dennis PB, Dennis PA, Deretic V, Devenish RJ, Di Sano F, Dice JF, Difiglia M, Dinesh-Kumar S, Distelhorst CW, Djavaheri-Mergny M, Dorsey FC, Dröge W, Dron M, Dunn WA Jr, Duszenko M, Eissa NT, Elazar Z, Esclatine A, Eskelinen EL, Fésüs L, Finley KD, Fuentes JM, Fueyo J, Fujisaki K, Galliot B, Gao FB, Gewirtz DA, Gibson SB, Gohla A, Goldberg AL, Gonzalez R, González-Estévez C, Gorski S, Gottlieb RA, Häussinger D, He YW, Heidenreich K, Hill JA, Høyer-Hansen M, Hu X, Huang WP, Iwasaki A, Jäättelä M, Jackson WT, Jiang X, Jin S, Johansen T, Jung JU, Kadowaki M, Kang C, Kelekar A, Kessel DH, Kiel JA, Kim HP, Kimchi A, Kinsella TJ, Kiselyov K, Kitamoto K, Knecht E, Komatsu M, Kominami E, Kondo S, Kovács AL, Kroemer G, Kuan CY, Kumar R, Kundu M, Landry J, Laporte M, Le W, Lei HY, Lenardo MJ, Levine B, Lieberman A, Lim KL, Lin FC, Liou W, Liu LF, Lopez-Berestein G, López-Otín C, Lu B, Macleod KF, Malorni W, Martinet W, Matsuoka K, Mautner J, Meijer AJ, Meléndez A, Michels P, Miotto G, Mistiaen WP, Mizushima N, Mograbi B, Monastyrska I, Moore MN, Moreira PI, Moriyasu Y, Motyl T, Münz C, Murphy LO, Naqvi NI, Neufeld TP, Nishino I, Nixon RA, Noda T, Nürnberg B, Ogawa M, Oleinick NL, Olsen LJ, Ozpolat B, Paglin S, Palmer GE, Papassideri I, Parkes M, Perlmutter DH, Perry G, Piacentini M, Pinkas-Kramarski R, Prescott M, Proikas-Cezanne T, Raben N, Rami A, Reggiori F, Rohrer B, Rubinsztein DC, Ryan KM, Sadoshima J, Sakagami H, Sakai Y, Sandri M, Sasakawa C, Sass M, Schneider C, Seglen PO, Seleverstov O, Settleman J, Shacka JJ, Shapiro IM, Sibirny A, Silva-Zacarin EC, Simon HU, Simone C, Simonsen A, Smith MA, Spanel-Borowski K, Srinivas V, Steeves M, Stenmark H, Stromhaug PE, Subauste CS, Sugimoto S, Sulzer D, Suzuki T, Swanson MS, Tabas I, Takeshita F, Talbot NJ, Tallóczy Z, Tanaka K, Tanaka K, Tanida I, Taylor GS, Taylor JP, Terman A, Tettamanti G, Thompson CB, Thumm M, Tolkovsky AM, Tooze SA, Truant R, Tumanovska LV, Uchiyama Y, Ueno T, Uzcátegui NL, van der Klei I, Vaquero EC, Vellai T, Vogel MW, Wang HG, Webster P, Wiley JW, Xi Z, Xiao G, Yahalom J, Yang JM, Yap G, Yin XM, Yoshimori T, Yu L, Yue Z, Yuzaki M, Zabirnyk O, Zheng X, Zhu X, Deter RL (2008) Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 4: 151-175

Iwai K, Koike M, Ohshima S, Miyatake K, Uchiyama Y, Saeki Y, Ishii M (2007) RGS18 acts as a negative regulator of osteoclastogenesis by modulating the acid-sensing OGR1/NFAT signaling pathway. J Bone Miner Res 22:1612-1620

Somboonthum F, Yoshii H, Okamoto S, Koie M, Gomi Y, Uchiyama Y, Takahashi M, Yamanishi K, Mori Y (2007) Generation of a recombinant Oka varicella vaccine expressing mumps virus hemagglutinin-neuraminidase protein as a polyvalent live vaccine. Vaccine 25:8741-8755

Hida T, Ikeda H, Kametaka S, Akazawa C, Kohsaka S, Ebisu S, Uchiyama Y, Waguri S (2007) Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells. Arch Histol Cytol 70:303-312

Tulone C, Uchiyama Y, Novelli Y, Grosvenor N, Saftig P, Chain BM (2007) Haematopoietic development and immunological function in the absence of cathepsin D. BMC Immunology 2007, 8:22 doi:10.1186/1471-2172-8-22

Miyanishi M, Tada K, Koike M, Uchiyama, Y, Kitamura T, Nagata S (2007) Identification of Tim-4 as a phosphatidylserine receptor. Nature 450: 435-439

Komatsu M, Waguri S, Koike M, Sou1 Y, Ueno T, Hara H, Mizushima N, Iwata J, Ezaki J, MurataS, Hamazaki J, Nishito Y, Iemura S, Natsume T, Yanagawa T, Uwayama J, Warabi E, Yoshida H, Ishii T, Kobayashi A, Yamamoto M, Yue Z, Uchiyama Y, Kominami E, Tanaka T (2007) Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131: 1149-1163

Yamasaki R, Zhang J, Koshiishi I, Suniarti DFS, Wu Z, Peters C, Schwake M, Uchiyama Y, Kira J, Saftig P, Utsumi H, Nakanishi H (2007) Involvement of lysosomal storage-induced p38 MAP kinase activation in the overproduction of nitric oxide by microglia in cathepsin D-deficient mice. Mol Cell Neurosci 35: 573-584

Yamanaka I, Koinuma S, Shigeyoshi Y, Uchiyama Y, Yagita K (2007) Presence of robust circadian clock oscillation under constitutive over-expression of mCry1 in rat-1 fibroblasts. FEBS Lett 581:4098-4102

Nakahara, M., Nagasaka, A., Koike, M., Uchida, K., Kawane, K., Uchiyama, Y., Nagata, S. (2007) Degradation of nuclear DNA by DNase II-like acid DNase in cortical fiber cells of mouse eye lens. FEBS J 274: 3055-3064

Komatsu, M, Ueno, T, Waguri, S, Uchiyama, Y, Kominami, E, Tanaka, K (2007) Constitutive autophagy: vital role in clearance of unfavorable proteins in neurons. Cell Death Diff 14:887-894

Shacka JJ, Klocke BJ, Young C, Shibata M, Olney JW, Uchiyama Y, Saftig P, Roth KA (2007) Cathepsin D Deficiency Induces Persistent Neurodegeneration in the Absence of Bax-Dependent Apoptosis. J Neurosci, 27: 2081-2090

Yagi R, Waguri S, Sumikawa S, Nada S, Oneyama C, Itami S, Schmedt C, Uchiyama Y, Okada M (2007) C-terminal Src kinase controls development and maintenance of mouse squamous epithelia. EMBO J 26: 1234-1244

Shacka JJ, Lu J, Xie ZL, Uchiyama Y, Roth KA, Zhang J. (2007) Kainic acid induces early and transient autophagic stress in mouse hippocampus. Neurosci Lett. 414: 57-60

Zhu, J-H, Horbinski, C., Guo, F., Watkins, S., Uchiyama, Y., Chu, C.T. (2006) Reguration of autophagy by extracellular signal regulated protein kinases during 1-methyl-4-phenylpyridinium injury. Am J Pathol 170:75-86

Waguri, S., Tomiyama, Y., Hida, T., Sakai, N., Taniike, M., Wakasugi, M., Ebisu, S., Uchiyama, Y. (2006) The luminal domain participates in the endosomal trafficking of the cation-independent mannose 6-phosphate receptor. Exp. Cell Res. 312: 4090-4107

Yamada S, Isojima Y, Kanamori S, Waguri S, Uchiyama Y, Nagai K. (2006) Uncoupling protein 2 negatively regulates neurite extensions in PC12h cells. Neurosci Lett. 410(2):110-114

Yae K, Keng VW, Koike M, Yusa K, Kouno M, Uno Y, Kondoh G, Gotow T, Uchiyama Y, Horie K, Takeda J. (2006) Sleeping beauty transposon-based phenotypic analysis of mice: lack of Arpc3 results in defective trophoblast outgrowth. Mol Cell Biol. 26: 6185-6196

Ishii M, Iwai K, Koike M, Ohshima S, Kudo-Tanaka E, Ishii T, Mima T, Katada Y, Miyatake K, Uchiyama Y, Saeki Y. (2006) RANKL-induced expression of tetraspanin CD9 in lipid raft membrane microdomain is essential for cell fusion during osteoclastogenesis. J Bone Miner Res. 21: 965-976

Yoshimura K, Shibata , Koike M, Gotoh K, Fukaya M, Watanabe M, Uchiyama Y (2006) Effects of RNA interference of Atg4B on the limited proteolysis of LC3 in PC12 cells and expression of Atg4B in various rat tissues. Autophagy 2: 200-208

Komatsu M, Waguri S, Chiba T, Murata S, Iwata J, Ueno Koike M, Uchiyama Y, Kominami E, Tanaka K (2006) Loss of autophagy in the central nervous system causes neurodegeneration. Nature 441: 880-884

Shimamura M, Sato N, Waguri S, Uchiyama Y, Hayashi T, Iida H, Nakamura T, Ogihara T, Kaneda Y, Morishita R (2006) Gene transfer of HGF gene improves learning and memory in the chronic stage of cerebral infarction. Hypertension 47: 742-751

Zhu C, Xu F, Wang X, Shibata M, Uchiyama Y, Blomgren K, Hagberg H (2006) Different apoptotic mechanisms are activated in male and female brains after neonatal hypoxia-ischaemia. J Neurochem, 96: 1016–1027

Shacka J, Klocke BJ, Shibata M, Uchiyama Y, Datta G, Schmidt RE, Roth KA (2006) Bafilomycin A1 Inhibits Chloroquine-Induced Death of Cerebellar Granule Neurons. Mol Pharacol 69: 1125-1136

Sasaki T, Gotow T, Shiozaki M, Sakaue F, Saito T, Julien JP, Uchiyama Y, Hisanaga S. (2006) Aggregate formation and phosphorylation of neurofilament-L Pro22 Charcot-Marie-Tooth disease mutants. Hum Mol Genet. 15:943-952

Hye-Won Shin, Hiromi Kobayashi, Masashi Kitamura, Satoshi Waguri, Tatsuo Suganuma, Yasuo Uchiyama, and Kazuhisa Nakayama (2005) Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking. J Cell Sci 118: 4039-4048

Koike M, Shibata M, Waguri S, Yoshimura K, Tanida I, Kominami E, Gotow G, Peters C, Figura Kv, Mizushima N, Saftig P and Uchiyama Y (2005) Participation of autophagy in storage of lysosomes in neurons from mouse models of neuronal ceroid-lipofuscinoses (Batten disease). Am J Pathol 167:1713-1728

Oonuki Y, Yoshida Y, Uchiyama Y, Asari A (2005) Application of fluorophore-assisted carbohydrate electrophoresis to analysis of disaccharides and oligosaccharides derived from glycosaminoglycans. Anal Biochem 343: 212-222

Yu WH, Cuervo AM, Kumar A, Peterhoff CM, Schmidt SD, Lee JH, Mohan PS, Mercken M, Farmery MR, Tjernberg LO, Jiang Y, Duff K, Uchiyama Y, J Näslund, Mathews PM, Cataldo AM, Nixon RA (2005) Macroautophagy－a novel amyloid-b (Ab) peptide-generating athway activated in alzheimer’s disease. J Cell Biol 171: 87-98

Takemoto M, Koike M, Mori Y, Yonemoto S, Sasamoto Y, Kondo K, Uchiyama Y, Yamanishi K (2005) Human herpesvirus 6 ORF14 protein and cellular p53 interact with each other and are contained in the virus. J Virol 79: 13037-13046

Yoshida H, Kawane K, Koike M, Mori Y, Uchiyama Y, Nagata, S (2005) Phosphatidylserine-dependent engulfment by macrophages of nuclei from erythroid precursor cells. Nature 434: 754-758

Shimizu, T., Hayashi, Y., Yamada, J., Zhang, J., Yamasaki, J., Ukai, K., Koike, M., Mine, K., Figura, K., Peters, C., Saftig, P., Fukuda, T., Uchiyama, Y., Nakanishi, H. (2005) Proteolytic Degradation of Glutamate Decarboxylase Mediates Disinhibition of Hippocampal CA3 Pyramidal Cells in Cathepsin D-deficient Mice. J Neurochem 94:680-690

Komatsu M, Waguri S, Ueno T, Iwata J, Murata S, Tanida I, Ezaki J, Mizushima N, Ohsumi Y, Uchiyama Y, Kominami E, Tanaka K, Chiba T (2005) Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol 169: 425–434

Lu Z, Dono K, Gotoh K, Shibata M, Koike M, Marubashi S, Miyamoto A, Takeda Y, Nagano H, Umeshita K, Uchiyama Y, Monden M (2005) Participation of autophagy in the degeneration process of rat hepatocytes after transplantation following prolonged cold preservation. Arch Histol Cytol 68: 71-80

Zhu C, Wang X, Xu F, Bahr BA, Shibata M, Uchiyama Y, Hagberg H, Blomgren K (2005) The influence of age on apoptotic and other mechanisms of cell death after cerebral hypoxia-ischemia. Cell Death Differ 12:162-176

Hashimoto N, Kido Y, Uchida T, Matsuda T, Suzuki K, Inoue H, Matsumoto M, Ogawa W, Maeda S, Uchiyama Y, Ohno S, Noda T, Kasuga M (2005) PKCλ in pancreatic β-cells mediates glucose-induced insulin secretion via the regulation of gene expression. J Clin Inv 115:138-145

Yu WH, Kumar A, Peterhoff C, Shapiro Kulnane L, Uchiyama Y, Lamb BT, Cuervo AM, Nixon RA (2004) Autophagic vacuoles are enriched in amyloid precursor protein-secretase activities: implications for beta-amyloid peptide over-production and localization in Alzheimer's disease. Int J Biochem Cell Biol 36: 2531-2540.

Tomiyama Y, Waguri S, Kanamori S, Kametaka S, Wakasugi M, Shibata M, Ebisu S, Uchiyama Y (2004) Early-phase redistribution of the cation-independent mannose 6-phosphate receptor by U18666A treatment in HeLa cells. Cell Tissue Res 317: 253-264

Hanayama R, Tanaka M, Miyasaka K, Aozasa K, Koike M, Uchiyama Y, Nagata S (2004) Autoimmune Disease and Impaired Uptake of Apoptotic Cells in MFG-E8-Deficient Mice. Science 304:1147-1150

Mori Y, Akkapaiboon P, Yonemoto S, Koike M, Takemoto M, Sadaoka T, Sasamoto Y, Konishi S, Uchiyama Y, Yamanishi K (2004) Discovery of a second form of tripartite complex containing gH-gL of human herpesvirus 6 and observations on CD46. J Virol 78:4609-4616

Yoshizaki T, Inaji M, Kouike H, Shimazaki T, Sawamoto K, Ando K, Date I, Kobayashi K, Suhara T, Uchiyama Y, Okano H. (2004) Isolation and transplantation of dopaminergic neurons generated from mouse embryonic stem cells. Neurosci Lett. 363:33-37

Shiba, T.,Shiba, Kametaka, S., Kawasaki, M., Shibata, M., Waguri, S., Uchiyama, Y., Wakatsuki, S. (2004) Insights into the phosphoregulation of beta-secretase sorting signal by the VHS domain of GGA1. Traffic 5:437-448

Shimamura, M., Sato, N., Ohshima, K., Aoki, M., Kurinami, H., Waguri, S., Uchiyama, Y., Ogiwara, T., Kaneda, Y., Morishita, R. (2004) Novel therapeutic strategy to treat brain ischemia overexpression of hepatocyte growth factor gene reduced ischemic injury without cerebral edema in rat model. Circulation 109: 424-431

Ezaki J, Takeda-Ezaki M, Koike M, Ohsawa Y, Taka H, Mineki R, Murayama K, Uchiyama Y, Ueno T, Kominami E (2003) Characterization of Cln3p, the gene product responsible for juvenile neuronal ceroid lipofuscinosis, as a lysosomal integral membrane glycoprotein. J Neurochem. 87:1296-1308

Mala V. Rao, Jabbar Campbell, Aidong Yuan, Asok Kumar, Takahiro Gotow, Yasuo Uchiyama, Ralph A. Nixon (2003) The Neurofilament middle molecular weight subunit carboxyl terminal tail domains are essential for the radial growth and cytoskeletal architecture of the axon but not for the regulation of rate of neurofilament transport. J. Cell Biol. 163: 1021-1031

Garcia, M.L., C. S. Lobsiger, S. B. Shah, T. J. Deerinck, J. Crum, D. Young, C. M. Ward, T. O. Crawford, T. Gotow, Y. Uchiyama, M. H. Ellisman, N. A. Calcutt, D. W. Cleveland (2003) NF-M is an essential target for the myelin-directed "outside in" signaling cascade that mediates radial axonal growth. J Cell Biol. 163: 1011-1020

Nishimoto, S., Kawane, K., Watanabe-Fukunaga, R., Fukuyama, H., Ohsawa, Y., Uchiyama, Y., Hashida, N., Ohguro, N., Tano, Y., Morimoto, T., Fukuda, Y., Nagata, S. (2003) Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens. Nature 424: 1071-1074.

Wakasugi M., Waguri S., Kametaka S., Tomiyama, Y., Kanamori, S., Shiba, Y., Nakayama, K., Uchiyama, Y. (2003) Predominant expression of the short form of GGA3 in human cell lines and tissues. Biochem Biophys Res Commun 306: 687-692

Yabuta, N., Kajimura, N., Mayanagi, K., Sato, M., Gotow, T., Uchiyama, Y., Ishimi, Y., Nojima, H. (2003) Mammalian Mcm2/4/6/7 complex forms a toroidal structure. Genes Cells 8:413-421

Kametaka S, Shibata M, Moroe K, Kanamori S, Ohsawa Y, Waguri S, Sims PJ, Emoto K, Umeda M, Uchiyama Y. (2003) Identification of phospholipid scramblase 1 as a novel interacting molecule with b -secretase (b-site amyloid precursor protein (APP) processing enzyme (BACE)). J Biol Chem. 278:15239-15245

Koike, M., Shibata, M., Ohsawa, Y., Nakanishi, H., Koga, T., Kametaka, S., Waguri, S., Momoi, T., Kominami, E., Peters, C., Figura, K.von, Saftig, S., Uchiyama, Y. (2003) Involvement of two different cell death pathways in retinal atrophy of cathepsin D-deficient mice. Mol. Cell. Neurosci. 14:142-155

Zhang, G., Ohsawa, Y., Kametaka, S., Shibata, M., Waguri, S., Uchiyama, Y. (2003) Regulation of FLRG expression in rat primary astroglial cells and injured brain tissue by transforming growth factor-beta1 (TGF-beta1). J. Neurosci. Res. 72: 33-45

Kawane, K., Fukuyama, H., Yoshida, H., Nagase H., Ohsawa, Y., Uchiyama, Y., Nagaya, S. (2003) Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation. Nat Immunol 4:138-144

Sakai, Y., Hosaka, M., Hira, Y., Harumi, T., Ohsawa, Y., Wang, H., Takeuchi, T., Uchiyama, Y., Watanabe, T. (2003) Immunocytochemical localization of secretogranin III in the anterior lobe of male rat pituitary glands. J. Histochem. Cytochem. 51: 227-238

Sakakibara, S., Nakamura, Y., Yoshida, T., Shibata, S., Koike, M., Takano, H., Ueda, S., Uchiyama, Y., Noda, T., Okano, H. (2003) RNA-binding protein Musashi family: Roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation. Proc. Natl. Acad. Sci. USA 99:15194-15199

Waguri, S., Dewitte, F., Borgne, R., Rouille, Y., Uchiyama, Y., Dubremetz, J.F., Hoflack, B. (2003) Visualization of TGN to endosomes trafficking through fluorescently labeled MPR and AP-1 in living cells. Mol. Biol. Cell 14: 142-155

Takahashi Y, Jeong SY, Ogata K, Goto J, Hashida H, Isahara K, Uchiyama Y, Kanazawa I. (2003) Human skeletal muscle calcium channel alpha1S is expressed in the basal ganglia: distinctive expression pattern among L-type Ca(2+) channels. Neurosci Res. 45:129-137

Nishimura, F., Naruishi, H., Naruishi, K., Yamada, T., Sasaki, J., Peters, C., Uchiyama, Y., Murayama, Y. (2002) Cathepsin L, a key molecule in the pathogenesis of drug-induced and I-cell disease-mediated gingival hyperplasia: a study with cathepsin L deficient mice. Am. J. Pathol. 161: 2047-2052

Hosaka, M., Watanabe, T., Sakai, Y., Uchiyama, Y., Takeuchi, T. (2002) Identification of a Chromogranin A Domain that Mediates Binding to Secretogranin III and Targeting to Secretory Granules in Pituitary Cells and Pancreatic b-Cells. Mol. Biol. Cell 13:3389-3399

Rao, M.V., Garcia, M.L., Miyazaki, Y., Gotow, T., Yuan, A., Mattina, S., Ward, C.M., Calcutt, N.A., Uchiyama, Y., Nixon, R.A., Cleaveland, D.W. (2002) Gene Replacement in mice reveals that the heavily phosphorylated tail of neurofilament heavy subunit (NF-H) does not affect axonal caliber or the transit of cargoes in slow axonal transport. J. Cell Biol. 158:681-93

Ogawa Y, Sawamoto K, Miyata T, Miyao S, Watanabe M, Nakamura M, Bregman BS, Koike M, Uchiyama Y, Toyama Y, Okano H. (2002) Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats. J Neurosci Res 69:925-933

Suzuki, H., Yanaka, A., Shibahara, T., Matsui, H., Nakahara, A., Tanaka, N., Muto, H., Momoi, T., Y. Uchiyama (2002) Ammonia-induced apoptosis is accelerated at higher pH in gastric surface mucous cells. Am. J. Physiol. Gastrointest. Liver Physiol. 283:G986-995

Koike, M., Shibata, M., Ohsawa, Y., Waguri, S., Kominami, E., Uchiyama, Y. (2002) Expression of tripeptidyl peptidase I in various tissues of rats and mice. Arch. Histol. Cytol. 65:219-232

Shinotsuka, C., Waguri, S., Wakasugi, S., Uchiyama, Y., Nakayama, K. (2002) Dominant negative mutant of BIG2, an ARF-guanin nucleotide exchange factor, specifically affects membrane trafficking from the trans-Golgi network through inhibiting membrane association of AP-1 and GGA coat proteins. Biochem. Biophys. Rep. Comm. 294: 254-260

Xu H, Ito T, Tawada A, Maeda H, Yamanokuchi H, Isahara K, Yoshida K, Uchiyama Y, Asari A (2002) Effect of hyaluronan oligosaccharides on the expression of heat shock protein 72. J Biol Chem 277:17308-17314

Yoshikawa, M., Uchida, S., Ezaki, J., Rai, T., Hayama, A., Kobayashi, K., Kida, Y., Noda, M., Koike, M., Uchiyama, Y., Marumo, F., Kominami, E., Sasaki, S. (2002) CLC-3 deficiency leads to phenotypes similar to human neuronal ceroid lipofuscinosis. Genes to Cells 7: 597-605

Shibata, M., Koike, M., Waguri, S., Zhang, G., Koga, T., Uchiyama, Y. (2002) Cathepsin D is specifically inhibited by deoxyribonucleic acids. FEBS Letters 517:281-284

Ariga K, Yonenobu K, Nakase T, Kaneko M, Okuda S, Uchiyama Y, Yoshikawa H. (2001) Localization of cathepsins D, K, and L in degenerated human intervertebral discs. Spine 26:2666-2672.

Kaneko, M., Tomita, T., Nakase, T., Ohsawa, Y., Seki, H., Takeuchi, E., Takano, H., Shi, K., Takahi, K., Kominami, E., Uchiyama, Y., Yoshikawa, H., Ochi, T. (2001) Expression of proteinases and inflammatory cytokines in subchondral bone regions in the destructive joint of rheumatoid arthritis. Rheumatology (Oxford) 40: 247-255

Hirabayashi, M., Inoue, K., Tanaka, K., Nakadate, K., Ohsawa, Y., Kamei. Y., Popiel, A.H., Sinohara, A., Iwamatsu, A., Kimura, Y., Uchiyama, Y., Hori, S., Kakizuka, A. (2001) VCP/p97 in abnormal protein aggregates, cytoplasmic vacuoles, and cell death, phenotypes relevant to neurodegeneration. Cell Death Differ 8: 977-984

Takahashi, K., Takeuchi, J., Takahashi, T., Miyauchi, S., Horie, K., Uchiyama, Y. (2001) Effects of sodium hyaluronate on epithelial healing of the vesical mucosa and vesical fibrosis in rabbits with acetic acid induced cystitis. J. Urology 166: 710-713

Waguri, S., Kohmura, M., Kanamori, S., Watanabe, T., Ohsawa, Y., Koike, M., Tomiyama, Y., Wakasugi, M., Kominami, E., Uchiyama, Y. (2001) Different distribution patterns of the two mannose 6-phosphate receptors in rat liver. J. Histochem. Cytochem. 49: 1397-1405

Nakanishi, H., Zang, J., Koike, M., Nishioku, T., Okamoto, Y., Kominami, E., Figura, K.v., Peters, C., Yamamoto, K., Saftig, P., Uchiyama, Y. (2001) Involvement of nitric oxide release from microglia/macrophages in pathological changes of cathepsin D-deficient mice. J. Neurosci. 21: 7526-7533

Yaginuma, H., Shiraiwa, N., Shimada, T., Nishiyama, K., Hong, J., Wang, S., Momoi, M., Uchiyama, Y., Oppenheim, R. (2001) Caspase activity is involved in, but is dispensable for, early motoneuron death in the chick embryo cervical spinal cord. Mol. Cell. Neurosci. 18: 168-182

Kawane, K., Fukuyama, H., Kondoh, G., Takeda, J., Ohsawa, Y., Uchiyama, Y., Nagata, S. (2001) Requirment of Dnase II for definitive erythropoiesis in the mouse fetal liver. Science 292: 1437-1596

Kawazoe, Y., Naka, T., Fujimoto, M., Kohzaki, H., Morita, Y., Narazaki, M., Okumura, K., Saitoh, H., Nakagawa, R., Uchiyama, Y., Akira, S., Kishimoto, T. (2001) SSI-1/SOCS1 inhibits insulin signal transduction pathway through modulating IRS-1 phosphorylation. J Exp. Med. 193:263-269

Shibata, M., Kanamori, S., Ohsawa, Y., Watanabe, T., Yayoi, Y., Miura, M., Uchiyama, Y. (2001) Prevention of Apoptosis of Mammalian Cells by the CED-3-Cleaved Form of CED-9. Arch. Histol. Cytol. 64: 17-28

Yayoi, Y., Ohsawa, Y., Koike, M., Zhang, G., Kominami, E., Uchiyama , Y. (2001) Specific Localization of Lysosomal Aminopeptidases in Type II Alveolar Epithelial Cells of Rat Lung. Arch. Histol. Cytol. 64: 89-97 IF: 1.503

Sapp, E., Kegel, K.B., Aronin, N., Hashikawa, T., Uchiyama, Y., Tohyama, K., Bhide, P.G., Vonsattel, J.P., DiFiglia, M (2001) Early and progressive accumulation of reactive microglia in the Huntington Disease brain. J. Neuropathol. Exp. Neurol. 60: 161-172

McIlroy, D., Tanaka, M., Sakahira, H., Fukuyama, H., Suzuki, M., Yamamura, K., Ohsawa, Y., Uchiyama Y., Nagata, S. (2000) Apoptotic DNA fragmentation by phagocytosis. Gene Dev 14:549-558 (88)

Chung, K.C., Park, J.H., Kim, C.H., Lee, H.W., Sato, N., Uchiyama, Y., Ahn, Y.S. (2000) Novel biphasic effect of pyrrolidine dithiocarbamate on neuronal cell viability is mediated by the differential regulation of intracellular zinc and copper ion levels, NF-kB, and MAP kinases. J. Neurosci. Res. 59: 117-125. (41)

Jeong, S., Goto, J., Hashida, H., Suzuki, T., Ogata, K., Masuda, N., Hirai, M., Isahara, K., Uchiyama, Y., Kanazawa, I. (2000) Identification of a novel human voltage-gated sodium channel a subunit gene, SCN12A. Biochem. Biophys. Res. Comm. 267: 262-270 (21)

Ogata, K., Jeong, S., Murakami, H., Hashida, H., Suzuki, T., Masuda, N., Hirai, M., Isahara, K., Uchiyama, Y., Goto, J., Kanazawa, I. (2000) Cloning and expression of study of the mouse tetrodotoxin-resistant voltage-gated sodium channel a subunit NaT/Scn11a. Biochem. Biophys. Res. Comm. 267: 271-277. (5)

Gotow, T., Shibata, M., Kanamori, S., Tokuno, O., Ohsawa, Y., Sato, N., Isahara, K., Yayoi, Y., Watanabe, T., Leterrier, J.F., Linden, M., Kominami, E., Uchiyama, Y. (2000) Selective localization of Bcl-2 to the inner mitochondrial and smooth endoplasmic reticul¨membranes in mammalian cells. Cell Death Diff. 7: 666-674 (24)

Haque, M., Chen, J., Ueda, K., Mori, Y., Nakano, K., Hirata, Y., Kanamori, S., Uchiyama, Y., Inagi, R., Okuno, T., Yamanishi, K. (2000) Identification and analysis of the K5 gene of Kaposi's sarcoma-associated herpesvirus. J. Virol. 74: 2867-75 (40)

Koike, M., Nakanishi, H., Saftig, P., Ezaki, J., Isahara, K., Ohsawa, Y., Schulz-Schaeffer, W., Watanabe, T., Waguri, W., Kametaka, S., Shibata, M., Yamamoto, K., Kominami, E., Peters, C., Figura, K., Uchiyama, Y. (2000) Cathepsin D deficiency induces lysosomal storage with ceroid lipofuscin in mouse CNS neurons. J. Neurosc. 20:6898-6906 (80)

Fujita, E., Urase, K., Egashira, J., Miho, Y., Isahara, K., Uchiyama, Y., Isoai, A., Kumagai, H., Kuida, K., Motoyama, N., Momoi, T. (2000) Detection of caspase-9 activation in the cell death of Bcl-x-deficient mouse embryo nervous system by cleavage sites-directed antisera. Brain Res. Dev. Brain Res. 122: 135-147 (18)

Sato, M., Gotow, T., You, Z., Komamura-Kohno, Y., Uchiyama, Y., Yabuta, N., Nojima, H., Ishimi, Y. (2000) Electron microscopic observation and single-stranded DNA binding activity of the Mcm4,6,7 complex. J. Mol. Biol. 300: 421-431 (48)

Matsumura, H., Ohsawa, Y., Kawahara, A., Uchiyama, Y., Nagata, S. (2000) Necrotic pathway in Fas receptor signaling. J. Cell Biol. 151:1247-1255 (99)

Nakase, T., Takeuchi, E., Sugimoto, K., Kaneko, M., Tomoi, T., Myoui, A., Uchiyama, Y., Ochi, T., Yoshikawa, H. (2000) Involvement of multinucleated giant cells synthetizing cathepsin K in calcified tendinitis of the rotator cuff tendons. Rheumatology 39: 1074-1077

Nakase, T. Kaneko, M., Tomita, T., Myoui, A., Ariga, K., Sugimoto, K., Uchiyama, Y., Ochi, T., Yoshikawa, H. (2000) Immunohistochemical detection of cathepsin D, K, and L in the process of endochondral ossification in the human. Histoch. Cell Biol. 114: 21-27 (26)

研究者

教育活動

【学校法人順天堂】研究者情報データベース

研究業績詳細

内山 安男（ウチヤマ ヤスオ）

内山安男（ウチヤマヤスオ）